口腔鳞状细胞癌的CD24 +肿瘤起始细胞在体内诱导初始血管生成。
CD24+ tumor-initiating cells from oral squamous cell carcinoma induce initial angiogenesis in vivo.
作者信息
Zimmerer Rüdiger M, Ludwig Nils, Kampmann Andreas, Bittermann Gido, Spalthoff Simon, Jungheim Michael, Gellrich Nils-Claudius, Tavassol Frank
机构信息
Department of Oral and Maxillofacial Surgery, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany.
Department of Oral and Maxillofacial Surgery, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany.
出版信息
Microvasc Res. 2017 Jul;112:101-108. doi: 10.1016/j.mvr.2017.03.006. Epub 2017 Mar 23.
BACKGROUND
In oral squamous cell carcinoma (OSCC), a minor subset of cancer stem cells has been identified using the surface marker CD24. The CD24+ cell population is involved in initiating, maintaining, and expanding tumor growth, but has not been reported to be involved in angiogenesis to date.
METHODS
NOD/SCID mice were equipped with dorsal skinfold chambers and gelatin sponges seeded with CD24+, CD24-, and unsorted cancer cells suspended in Matrigel® were implanted. Following intravital fluorescence microscopy, specimens were examined by immunohistology.
RESULTS
Sponges seeded with CD24+ cells showed a significantly higher functional capillary density than those seeded with CD24- cells. The presence of endothelial cells was confirmed by immunohistochemistry for CD31.
CONCLUSION
For the first time, CD24+ tumorigenic cells with angiogenic potential, which were isolated from OSCC, were characterized. Our findings provide a promising in vivo model to facilitate the development of therapeutic agents against cancer stem cells and their angiogenic pathways.
背景
在口腔鳞状细胞癌（OSCC）中，已利用表面标志物CD24鉴定出一小部分癌症干细胞。CD24+细胞群体参与启动、维持和扩大肿瘤生长，但迄今为止尚未有报道表明其参与血管生成。
方法
给NOD/SCID小鼠配备背部皮褶小室，并植入接种有悬浮于基质胶中的CD24+、CD24-和未分选癌细胞的明胶海绵。在活体荧光显微镜检查后，通过免疫组织学检查标本。
结果
接种CD24+细胞的海绵显示出比接种CD24-细胞的海绵显著更高的功能性毛细血管密度。通过针对CD31的免疫组织化学证实了内皮细胞的存在。
结论
首次对从OSCC中分离出的具有血管生成潜力的CD24+致瘤细胞进行了表征。我们的研究结果提供了一个有前景的体内模型，以促进针对癌症干细胞及其血管生成途径的治疗药物的开发。
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