Schütze S, Scheurich P, Schlüter C, Ucer U, Pfizenmaier K, Krönke M
Clinical Research Group Biological Regulation of Host-Tumor-Interaction, Max-Planck-Society, Göttingen, Federal Republic of Germany.
J Immunol. 1988 May 1;140(9):3000-5.
TNF-alpha alone or in combination with IFN-gamma differentially affects the proliferation and differentiation of the human leukemic cell line U937 and two derivatives C27 and G3. All three cell lines express similar numbers of functional, high affinity receptors for both TNF-alpha and IFN-gamma. In C27 and G3 cells, TNF-alpha as well as IFN-gamma induced changes in steady state levels of specific mRNA, which appear to be associated with TNF-alpha and IFN-gamma diverse effects on cell growth and differentiation. Constitutive differences in membrane phosphorylation patterns suggest that altered transduction of TNF-alpha signals may account for the differential response of these three cell lines. Several lines of evidence indicate that C27 and G3 cells, when compared with parental U937 cells represent discretely higher stages of monocytic differentiation, suggesting that cellular differentiation may contribute to the development of resistance to the action of TNF-alpha.
单独的肿瘤坏死因子-α（TNF-α）或其与干扰素-γ（IFN-γ）联合使用对人白血病细胞系U937及其两个衍生物C27和G3的增殖和分化有不同影响。这三种细胞系表达相似数量的针对TNF-α和IFN-γ的功能性高亲和力受体。在C27和G3细胞中，TNF-α以及IFN-γ诱导了特定mRNA稳态水平的变化，这似乎与TNF-α和IFN-γ对细胞生长和分化的不同作用相关。膜磷酸化模式的组成性差异表明，TNF-α信号转导的改变可能解释了这三种细胞系的不同反应。多条证据表明，与亲本U937细胞相比，C27和G3细胞代表单核细胞分化的离散更高阶段，这表明细胞分化可能有助于对TNF-α作用产生抗性。
