骨髓间充质干细胞对重塑小鼠脂多糖诱导的肺损伤的影响。
Impact of bone marrow-derived mesenchymal stem cells on remodeling the lung injury induced by lipopolysaccharides in mice.
作者信息
Mohi El-Din Mouchira M, Rashed Laila A, Mahmoud Haridy Mohi A, Khalil Atef Mohamed, Mohamed Albadry Mohamed A
机构信息
Pathology & Clinical Pathology Department, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt; Pathology & Clinical Pathology Department, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt.
Biochemistry & Molecular Biology Department, Medicine Faculty, Cairo University, Cairo, Egypt; Biochemistry & Molecular Biology Department, Medicine Faculty, Cairo University, Cairo, Egypt.
出版信息
Future Sci OA. 2017 Jan 17;3(1):FSO162. doi: 10.4155/fsoa-2016-0036. eCollection 2017 Mar.
AIM
This study evaluated the potential of bone marrow derived mesenchymal stem cells (MSCs) to regulate cytokines and remodel the lung induced by lipopolysaccharide (LPS; O-antigen).
MATERIALS & METHODS: A group of mice (n = 21) was inoculated intraperitoneally with one dose 0.1 ml containing 0.025 mg LPS/mouse, and another treated intravenously with one dose of labeling bone marrow derived MSCs at 7.5 × 10 cell/mouse 4 h after LPS injection. All animals were sacrificed on the 1st, 7th and 14th days post-injection.
RESULTS
MSCs increased the level of IL-10 with suppression of TNF-α, decrease of collagen fibers and renewal of alveolar type I cells, together with lung tissue remodeling.
CONCLUSION
MSCs were shown to modulate inflammatory cytokines (TNF-α and IL-10) and to differentiate into alveolar type I cells, which prevented fibrosis in lung tissue from LPS-treated mice.
目的
本研究评估了骨髓间充质干细胞（MSCs）调节细胞因子以及重塑脂多糖（LPS；O抗原）诱导的肺组织的潜力。
材料与方法
一组小鼠（n = 21）腹腔注射一剂0.1 ml含0.025 mg LPS/小鼠，另一组在LPS注射后4小时静脉注射一剂标记的骨髓间充质干细胞，剂量为7.5×10⁶细胞/小鼠。所有动物在注射后第1、7和14天处死。
结果
间充质干细胞增加了IL-10水平，抑制了TNF-α，减少了胶原纤维并使I型肺泡细胞更新，同时伴有肺组织重塑。
结论
间充质干细胞被证明可调节炎性细胞因子（TNF-α和IL-10）并分化为I型肺泡细胞，从而预防LPS处理小鼠的肺组织纤维化。
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