Guillerault N, Bouletreau S, Iribar A, Valentini A, Santoul F
EcoLab, Université de Toulouse, CNRS, INPT, UPS, 31062, Toulouse, France.
Station d'Ecologie Expérimental du CNRS à Moulis, Lab. USR 2936, 09 100, Moulis, France.
J Fish Biol. 2017 May;90(5):2214-2219. doi: 10.1111/jfb.13294. Epub 2017 Mar 27.
In this study, the results of conventional stomach-content analysis are compared with the recent DNA metabarcoding approach on faeces to identify fish species consumed by non-native European catfish Silurus glanis in the Garonne River (south-western France), with a special emphasis on anadromous prey. Fourteen prey species were identified in the stomach contents or faeces, including four anadromous fish species. Despite higher intestine than stomach emptiness, more species were identified through faecal analysis (11 of 14) than through stomach-content analysis (five of 14) suggesting that DNA metabarcoding on faeces is an efficient, non-intrusive technique to study the diet of predatory fishes.
在本研究中，将传统胃内容物分析的结果与最近粪便DNA元条形码方法的结果进行比较，以识别法国西南部加龙河中非本地欧洲六须鲶（Silurus glanis）所捕食的鱼类物种，特别关注溯河产卵的猎物。在胃内容物或粪便中鉴定出14种猎物物种，包括4种溯河产卵鱼类。尽管肠道比胃的排空程度更高，但通过粪便分析鉴定出的物种（14种中的11种）比通过胃内容物分析鉴定出的物种（14种中的5种）更多，这表明粪便DNA元条形码技术是研究掠食性鱼类食性的一种有效、非侵入性技术。
