Karnup S V, Kolomoets A V
Neirofiziologiia. 1987;19(6):816-24.
Background neuronal discharges were recorded extracellularly in guinea pig cerebral cortex slices maintained in vitro. Synaptic disconnection was reached by adding 100 microM of adenosine or 20-75 microM of 5-AMP. It was shown that 30-40% of neurons continued to discharge even under conditions of complete synaptic blockade, although the discharge frequency decreased. These neurons were considered as endogenously active ones. Other cells stopped their activity as soon as adenosine reached the slice, but they began to generate action potentials again after superfusion with normal solution. These cells were synaptically activated and were considered as exogenously active. In particular most cells with initially very regular discharges became silent during blockade and restored their activity after superfusion with normal saline. This fact suggests the existence of reverberating activity in neocortical slices with periods of 30-140 ms.
在体外培养的豚鼠大脑皮质切片中，细胞外记录背景神经元放电。通过添加100微摩尔的腺苷或20 - 75微摩尔的5 - 腺苷酸达到突触断开。结果表明，即使在完全突触阻断的条件下，仍有30 - 40%的神经元继续放电，尽管放电频率降低。这些神经元被认为是内源性活动神经元。其他细胞在腺苷进入切片后立即停止活动，但在用正常溶液灌流后又开始产生动作电位。这些细胞是突触激活的，被认为是外源性活动神经元。特别是大多数最初放电非常规则的细胞在阻断期间变得沉默，并在用生理盐水灌流后恢复其活动。这一事实表明新皮质切片中存在周期为30 - 140毫秒的回响活动。
