d'Argy R, Gillberg P G, Stålnacke C G, Persson A, Bergström M, Långström B, Schoeps K O, Aquilonius S M
Department of Toxicology, Uppsala University, Sweden.
Neurosci Lett. 1988 Mar 10;85(3):304-10. doi: 10.1016/0304-3940(88)90583-6.
In vitro autoradiography of an 11C-labelled ligand, Ro 15-1788, has been used to visualize benzodiazepine binding sites in large human brain cryo-sections. In parallel, using the same radioligand, an in vivo study of a human healthy volunteer was performed, by means of positron emission tomography (PET). The in vitro and in vivo mapping of the ligand demonstrated a very similar binding pattern, although the poor resolution of PET precluded a full discrimination of fine details. The 11C-autoradiograms showed good spatial resolution, even with distinction of different layers in the cerebral and cerebellar cortex. In a separate experiment, the spatial resolution of 11C-autoradiography was found to be 180 microns, using 80-microns-thick cryo-sections. It is emphasized that in vitro and in vivo studies of the same radioligand give complementary information, which is valuable in the assessment of PET images.
利用¹¹C标记的配体Ro 15 - 1788进行体外放射自显影，已用于在人大脑冷冻切片中可视化苯二氮䓬结合位点。同时，使用相同的放射性配体，通过正电子发射断层扫描（PET）对一名健康志愿者进行了体内研究。尽管PET分辨率较差，无法完全区分精细细节，但配体的体外和体内图谱显示出非常相似的结合模式。¹¹C放射自显影片显示出良好的空间分辨率，甚至能区分大脑和小脑皮质的不同层。在另一个实验中，使用80微米厚的冷冻切片，发现¹¹C放射自显影的空间分辨率为180微米。需要强调的是，对同一放射性配体进行体外和体内研究可提供互补信息，这对评估PET图像很有价值。
