Nicolucci Carla, Errico Sonia, Federico Alessandro, Dallio Marcello, Loguercio Carmelina, Diano Nadia
Department of Experimental Medicine, University of Campania "L. Vanvitelli", Via S. M. di Costantinopoli, 16, 80138 Naples, Italy.
Department of Clinical and Experimental Medicine, Division of Hepatogastroenterology, University of Campania "L. Vanvitelli", Via Pansini, 5, 80131 Naples, Italy.
J Pharm Biomed Anal. 2017 Jun 5;140:105-112. doi: 10.1016/j.jpba.2017.02.058. Epub 2017 Mar 10.
A selective and highly sensitive analytical methodology for determination of Bisphenol A in human plasma was developed and validated. The method was based on selective liquid/solid extraction, combined with liquid chromatography-electrospray ionization tandem mass spectrometry in the multiple reaction monitoring mode and negative ionization. The linearity of the detector response was verified in human plasma over the concentration range 0.100-200ngmL. The detection limit was 0.03ngmL and the quantification limit was 0.100ngmL. The analytical features of the proposed in-house validated method were satisfactory: precision was <10% and recoveries were around 84-104%. The matrix effect was studied and compensated using deuterated labeled standard. The applicability of the proposed method was demonstrated analyzing human plasma samples from individuals affected by non-alcoholic fatty liver disease. Bisphenol A was detected above the detection limit in all samples. The data show a persistence of unconjugated Bisphenol A levels in plasma and indicate a chronic Bisphenol A exposure of the target organ, suggesting an association between liver health status and Bisphenol A exposure. The results from our study are valuable for further investigation with large sample size and longitudinal study designs, necessary to confirm the observed association.
开发并验证了一种用于测定人血浆中双酚A的选择性高灵敏度分析方法。该方法基于选择性液/固萃取，结合液相色谱-电喷雾电离串联质谱的多反应监测模式和负离子化。在人血浆中，检测器响应的线性在0.100 - 200ng/mL的浓度范围内得到验证。检测限为0.03ng/mL，定量限为0.100ng/mL。所提出的内部验证方法的分析特性令人满意：精密度<10%，回收率约为84 - 104%。使用氘代标记标准物研究并补偿了基质效应。通过分析非酒精性脂肪性肝病患者的人血浆样本，证明了所提出方法的适用性。在所有样本中均检测到双酚A高于检测限。数据显示血浆中未结合双酚A水平持续存在，表明靶器官长期暴露于双酚A，提示肝脏健康状况与双酚A暴露之间存在关联。我们的研究结果对于进一步开展大样本量和纵向研究设计的调查很有价值，这对于确认观察到的关联是必要的。
