Wiik P, Opstad P K, Knardahl S, Bøyum A
Norwegian Defence Research Establishment, Division for Environmental Toxicology, Kjeller.
Peptides. 1988 Jan-Feb;9(1):181-6. doi: 10.1016/0196-9781(88)90025-3.
VIP receptors on blood mononuclear leucocytes and plasma VIP concentrations were studied during a ranger training course lasting for five days with almost continuous physical activity, and energy deficiency. The maximum binding capacity (Bmax) for the high affinity receptor increased (p less than 0.0005) from 0.71 (SEM = 0.11, N = 10) fmol/million cells to a maximum of 7.33 (SEM = 1.0) fmol/million cells on Day 4. There was no significant change in the dissociation constant (Kd) for the high affinity receptor, and no effect on Kd nor Bmax for the low affinity VIP receptor was detected. Plasma VIP concentration increased (p less than 0.0005) from 8.8 pmol/l (SEM = 0.6) to a maximum of 23.4 (SEM = 1.9) on the second day of the course. However, the highest plasma concentrations were about one order of magnitude lower than the dissociation constant (Kd) for the high affinity VIP receptor on the mononuclear leucocytes. These data indicate that heterologous upregulation of the high affinity VIP receptor on mononuclear blood cells takes place during combined strenuous physical exercise, and calorie deficiency.
在一个为期五天、几乎持续进行体育活动且存在能量不足的 ranger 训练课程期间，研究了血液单核白细胞上的 VIP 受体和血浆 VIP 浓度。高亲和力受体的最大结合容量（Bmax）从 0.71（标准误 = 0.11，N = 10）fmol/百万细胞增加（p < 0.0005）至第 4 天的最高值 7.33（标准误 = 1.0）fmol/百万细胞。高亲和力受体的解离常数（Kd）无显著变化，且未检测到对低亲和力 VIP 受体的 Kd 及 Bmax 有影响。血浆 VIP 浓度在课程第二天从 8.8 pmol/l（标准误 = 0.6）增加（p < 0.0005）至最高值 23.4（标准误 = 1.9）。然而，血浆最高浓度比单核白细胞上高亲和力 VIP 受体的解离常数（Kd）低约一个数量级。这些数据表明，在剧烈体育活动和热量不足共同作用期间，单核血细胞上高亲和力 VIP 受体发生了异源上调。
