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聚合物包覆金纳米粒子与抗体的偶联——合成与表征

Conjugation of Polymer-Coated Gold Nanoparticles with Antibodies-Synthesis and Characterization.

作者信息

Tan Gamze, Kantner Karsten, Zhang Qian, Soliman Mahmoud G, Del Pino Pablo, Parak Wolfgang J, Onur Mehmet A, Valdeperez Daniel, Rejman Joanna, Pelaz Beatriz

机构信息

Faculty of Science and Letters, Department of Biology, Aksaray University, Aksaray 68100,Turkey.

Philipp University of Marburg, Marburg 35001, Germany.

出版信息

Nanomaterials (Basel). 2015 Aug 7;5(3):1297-1316. doi: 10.3390/nano5031297.

DOI:10.3390/nano5031297
PMID:28347065
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5304631/
Abstract

The synthesis of polymer-coated gold nanoparticles with high colloidal stability is described, together with appropriate characterization techniques concerning the colloidal properties of the nanoparticles. Antibodies against vascular endothelial growth factor (VEGF) are conjugated to the surface of the nanoparticles. Antibody attachment is probed by different techniques, giving a guideline about the characterization of such conjugates. The effect of the nanoparticles on human adenocarcinoma alveolar basal epithelial cells (A549) and human umbilical vein endothelial cells (HUVECs) is probed in terms of internalization and viability assays.

摘要

本文描述了具有高胶体稳定性的聚合物包覆金纳米颗粒的合成方法，以及有关纳米颗粒胶体性质的适当表征技术。将抗血管内皮生长因子（VEGF）抗体偶联到纳米颗粒表面。通过不同技术探测抗体附着情况，为这类偶联物的表征提供了指导。通过内化和活力测定来探究纳米颗粒对人肺腺癌肺泡基底上皮细胞（A549）和人脐静脉内皮细胞（HUVECs）的影响。

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