阿尔及利亚人群中芳胺N-乙酰基转移酶2基因多态性
Arylamine N-acetyltransferase 2 gene polymorphism in an Algerian population.
作者信息
Chelouti Hiba, Khelil Malika
机构信息
a Département de Biologie Cellulaire et Moléculaire, Faculté des Sciences Biologiques, Université des Sciences et de la Technologie Houari Boumediene , Alger ; Algérie.
出版信息
Ann Hum Biol. 2017 Sep;44(6):531-536. doi: 10.1080/03014460.2017.1311373. Epub 2017 Apr 18.
BACKGROUND
The arylamine N-acetyltransferase 2 (NAT2) is a key enzyme in the biotransformation of xenobiotics. NAT2 gene polymorphisms have been associated with the risk of isoniazid hepatotoxicity and these polymorphisms change among different populations.
AIM
The objective of this study is to investigate NAT2 polymorphisms in order to predict the prevalence of NAT2 phenotype in an Algerian population.
SUBJECTS AND METHODS
Genotyping of NAT2 was done using a PCR-RFLP method. Haplotype was analysed using the software package PHASE, version 2.0.
RESULTS
The major haplotypes were NAT25B (23.72%), NAT26 A (18.61%), NAT24 (14.60%) and NAT25 F (10%). The average of the expected slow acetylator phenotype was 53%.
CONCLUSION
Our results suggest that the high frequency of slow acetylator phenotype requires investigation into its possible association with ATDH.
背景
芳胺N - 乙酰基转移酶2（NAT2）是外源性物质生物转化中的关键酶。NAT2基因多态性与异烟肼肝毒性风险相关，且这些多态性在不同人群中有所变化。
目的
本研究旨在调查NAT2多态性，以预测阿尔及利亚人群中NAT2表型的流行情况。
对象与方法
采用PCR - RFLP方法对NAT2进行基因分型。使用PHASE 2.0软件包分析单倍型。
结果
主要单倍型为NAT25B（23.72%）、NAT26A（18.61%）、NAT24（14.60%）和NAT25F（10%）。预期慢乙酰化表型的平均比例为53%。
结论
我们的结果表明，慢乙酰化表型的高频率需要研究其与ATDH可能的关联。
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