Li Bo, Li Bai, Sun Hongyan, Zhang Haishan
1 Department of Gastrointestinal Colorectal and Anal Surgery, China-Japan Union Hospital of Jilin University, Changchun, P.R. China.
2 Department of Colorectal and Anal Surgery, The First Affiliated Hospital of Jilin University, Changchun, P.R. China.
Tumour Biol. 2017 Mar;39(3):1010428317692257. doi: 10.1177/1010428317692257.
MicroRNAs are known as small, non-coding, and single-stranded RNAs which can regulate cell proliferation, differentiation, and apoptosis and involve in the development of tumors. In this study, colorectal cancer tissue morphological change in different prognosis in patients was observed by hematoxylin and eosin staining. Thereafter, differentially expressed miR-22 and TIAM1 gene were detected using quantitative polymerase chain reaction and western blot in different colorectal cancer tissues. Meanwhile, luciferase reporter gene system was used to verify the relationship between miR-22 and TIAM1. Eventually, the survival curve was plotted according to follow-up records of patients with colorectal cancer and the expression levels of miR-22 and TIAM1 in different tumor tissues. The hematoxylin and eosin results showed the poor pathological features in the 1-year survival group. The expression level of miR-22 was upregulated and TIAM1 was inhibited, correlating with the extension of patients' survival time. Our results indicated that miR-22 and TIAM1 might play a regulatory role in the occurrence and development of colorectal cancer which were consistent with the survival curve analysis results. Furthermore, the luciferase in miR-22 co-transfected with pmiR-RB-REPORT- TIAM1 group was significantly lower than pmiR-RB-REPORT- TIAM1-mut and Si groups. Collectively, these data suggest that miR-22 may suppress the expression of its target gene TIAM1. The low miR-22 level or the high TIAM1 level will indicate the poor prognosis in colorectal cancer patients.
微小RNA是一类小的、非编码的单链RNA，可调节细胞增殖、分化和凋亡，并参与肿瘤的发生发展。在本研究中，通过苏木精-伊红染色观察了不同预后患者的结直肠癌组织形态学变化。此后，采用定量聚合酶链反应和蛋白质印迹法检测不同结直肠癌组织中差异表达的miR-22和TIAM1基因。同时，利用荧光素酶报告基因系统验证miR-22与TIAM1之间的关系。最终，根据结直肠癌患者的随访记录以及不同肿瘤组织中miR-22和TIAM1的表达水平绘制生存曲线。苏木精-伊红染色结果显示1年生存组的病理特征较差。miR-22表达水平上调，TIAM1受到抑制，这与患者生存时间的延长相关。我们的结果表明，miR-22和TIAM1可能在结直肠癌的发生发展中发挥调节作用，这与生存曲线分析结果一致。此外，与pmiR-RB-REPORT-TIAM1-mut和Si组共转染miR-22的荧光素酶活性显著低于pmiR-RB-REPORT-TIAM1组。综上所述，这些数据表明miR-22可能抑制其靶基因TIAM1的表达。miR-22水平低或TIAM1水平高表明结直肠癌患者预后较差。
