密码子72处p53精氨酸/精氨酸纯合基因型与人类乳头瘤病毒E6/E7信使核糖核酸表达之间的关联。

Association between the p53 arginine/arginine homozygous genotype at codon 72 and human papillomavirus E6/E7 mRNA expression.

作者信息

Chuery Ana Carolina Silva, Silva Ismael Dale Cotrim Guerreiro da, Ribalta Julisa Chamorro Lascasas, Speck Neila Maria de Góis

机构信息

Universidade Federal de São Paulo (Unifesp), Departamento de Ginecologia, Núcleo de Prevenção de Doenças Ginecológicas, São Paulo, SP, Brazil.

Universidade Federal de São Paulo (Unifesp), Departamento de Ginecologia, Escola Paulista de Medicina, São Paulo, SP, Brazil.

出版信息

Braz J Infect Dis. 2017 May-Jun;21(3):248-254. doi: 10.1016/j.bjid.2017.03.002. Epub 2017 Mar 25.

DOI:10.1016/j.bjid.2017.03.002

PMID:28347732

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9428040/

Abstract

OBJECTIVE

To evaluate the association between p53 polymorphisms and human papillomavirus (HPV) E6/E7 mRNA expression.

METHODS

We analyzed 175 cervical samples from women aged 16-69 years old who were tested for HPV E6/E7 mRNA expression (NucliSENS EasyQ HPV). The samples were divided into three groups: positive (n=75) those with positive HPV E6/E7 mRNA expression and positive high-risk HPV Hybrid Capture (HR-HC) test; negative (n=52) those with negative HPV E6/E7 mRNA expression and positive HR-HC; and control (n=48) those with negative HPV E6/E7 mRNA expression and negative HR-HC. The p53 polymorphisms at codons 11, 72, and 248 were evaluated through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).

RESULTS

The frequency of the arginine/arginine homozygous genotype at codon 72 was significantly higher in the positive (49.3%) than in the negative (32.7%) and control groups (20.8%, p=0.002*). The frequency of the arginine allele was also significantly higher in the positive (67.3%) than in the negative (53.8%) and control groups (38.5%, p<0.001*). The arginine/arginine homozygous genotype was significantly associated with positive HPV E6/E7 mRNA expression (positive group) compared with negative and control groups (odds ratio: 2.633; 95% CI, 1.399-4.954, p=0.003). The frequency of arginine/arginine homozygous genotype at codon 72 remained significantly more frequent in the positive group of women aged ≥30 years than in the other two groups.

CONCLUSION

The presence of the p53 arginine/arginine homozygous genotype at codon 72 was significantly associated with the positive HPV E6/E7 mRNA expression.

摘要

目的

评估p53基因多态性与人类乳头瘤病毒（HPV）E6/E7 mRNA表达之间的关联。

方法

我们分析了16至69岁女性的175份宫颈样本，这些样本均进行了HPV E6/E7 mRNA表达检测（NucliSENS EasyQ HPV）。样本被分为三组：阳性组（n=75），HPV E6/E7 mRNA表达阳性且高危HPV杂交捕获（HR-HC）检测呈阳性；阴性组（n=52），HPV E6/E7 mRNA表达阴性但HR-HC检测呈阳性；对照组（n=48），HPV E6/E7 mRNA表达阴性且HR-HC检测呈阴性。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）评估第11、72和248位密码子处的p53基因多态性。

结果

第72位密码子处精氨酸/精氨酸纯合基因型的频率在阳性组（49.3%）显著高于阴性组（32.7%）和对照组（20.8%，p=0.002*）。精氨酸等位基因的频率在阳性组（67.3%）也显著高于阴性组（53.8%）和对照组（38.5%，p<0.001*）。与阴性组和对照组相比，精氨酸/精氨酸纯合基因型与HPV E6/E7 mRNA表达阳性（阳性组）显著相关（优势比：2.633；95%可信区间，1.399-4.954，p=0.003）。在年龄≥30岁的女性阳性组中，第72位密码子处精氨酸/精氨酸纯合基因型的频率仍显著高于其他两组。