Department of Lipidomics, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; Life Sciences Core Facility, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Department of Lipidomics, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Biochim Biophys Acta Mol Cell Biol Lipids. 2017 Aug;1862(8):777-781. doi: 10.1016/j.bbalip.2017.03.008. Epub 2017 Mar 27.
Comprehensive quantitative analysis of lipid mediators using liquid chromatography-tandem mass spectrometry is an effective strategy in the elucidation of disease mechanisms; but technically, it has been and is still a great challenge to achieve reliable datasets that cover variety of lipid metabolites contained at trace levels in complex biological matrices. In this opinion article, we introduce our experiences in developing lipid mediator profiling systems, and deliver some comments on limitations of current methodology.
采用液相色谱-串联质谱技术对脂质介质进行全面定量分析是阐明疾病机制的有效策略；但从技术上讲，要获得涵盖复杂生物基质中痕量水平的多种脂质代谢物的可靠数据集，一直以来都是一个巨大的挑战。在本文观点中，我们介绍了开发脂质介质分析系统的经验，并对当前方法学的局限性提出了一些看法。
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