Dalli Jesmond, Colas Romain A, Walker Mary E, Serhan Charles N
Lipid Mediator Unit, Biochemical Pharmacology, William Harvey Research Institute, Barts and the London School of Medicine, Queen Mary University of London, London, UK.
Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Methods Mol Biol. 2018;1730:59-72. doi: 10.1007/978-1-4939-7592-1_4.
Solid-phase extraction coupled with liquid chromatography tandem mass spectrometry provides a robust and sensitive approach for the identification and quantitation of specialized pro-resolving mediators (lipoxins, resolvins, protectins, and maresins), their pathway markers and the classic eicosanoids. Here, we provide a detailed description of the methodologies employed for the extraction of these mediators from biological systems, setup of the instrumentation, sample processing, and then the procedures followed for their identification and quantitation.
固相萃取结合液相色谱串联质谱法为鉴定和定量特殊促消退介质（脂氧素、消退素、保护素和maresin）、其信号通路标志物以及经典类二十烷酸提供了一种强大而灵敏的方法。在此，我们详细描述了从生物系统中提取这些介质、仪器设置、样品处理以及随后用于其鉴定和定量的程序所采用的方法。
