姜黄素通过抑制CD8CD11c细胞对实验性结肠炎的治疗作用。
Therapeutic effect of curcumin on experimental colitis mediated by inhibiting CD8CD11c cells.
作者信息
Zhao Hai-Mei, Han Fei, Xu Rong, Huang Xiao-Ying, Cheng Shao-Min, Huang Min-Fang, Yue Hai-Yang, Wang Xin, Zou Yong, Xu Han-Lin, Liu Duan-Yong
机构信息
Hai-Mei Zhao, Shao-Min Cheng, School of Basic Medical Sciences, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, Jiangxi Province, China.
出版信息
World J Gastroenterol. 2017 Mar 14;23(10):1804-1815. doi: 10.3748/wjg.v23.i10.1804.
AIM
To verify whether curcumin (Cur) can treat inflammatory bowel disease by regulating CD8CD11c cells.
METHODS
We evaluated the suppressive effect of Cur on CD8CD11c cells in spleen and Peyer's patches (PPs) in colitis induced by trinitrobenzene sulfonic acid. Mice with colitis were treated by 200 mg/kg Cur for 7 d. On day 8, the therapeutic effect of Cur was evaluated by visual assessment and histological examination, while co-stimulatory molecules of CD8CD11c cells in the spleen and PPs were measured by flow cytometry. The levels of interleukin (IL)-10, interferon (IFN)-γ and transforming growth factor (TGF)-β1 in spleen and colonic mucosa were determined by ELISA.
RESULTS
The disease activity index, colon weight, weight index of colon and histological score of experimental colitis were obviously decreased after Cur treatment, while the body weight and colon length recovered. After treatment with Cur, CD8CD11c cells were decreased in the spleen and PPs, and the expression of major histocompatibility complex II, CD205, CD40, CD40L and intercellular adhesion molecule-1 was inhibited. IL-10, IFN-γ and TGF-β1 levels were increased compared with those in mice with untreated colitis.
CONCLUSION
Cur can effectively treat experimental colitis, which is realized by inhibiting CD8CD11c cells.
目的
验证姜黄素（Cur）是否可通过调节CD8⁺CD11c⁺细胞来治疗炎症性肠病。
方法
我们评估了Cur对三硝基苯磺酸诱导的结肠炎小鼠脾脏和派尔集合淋巴结（PPs）中CD8⁺CD11c⁺细胞的抑制作用。用200mg/kg Cur治疗结肠炎小鼠7天。在第8天，通过视觉评估和组织学检查评估Cur的治疗效果，同时通过流式细胞术检测脾脏和PPs中CD8⁺CD11c⁺细胞的共刺激分子。通过酶联免疫吸附测定法（ELISA）测定脾脏和结肠黏膜中白细胞介素（IL）-10、干扰素（IFN）-γ和转化生长因子（TGF）-β1的水平。
结果
Cur治疗后，实验性结肠炎的疾病活动指数、结肠重量、结肠重量指数和组织学评分明显降低，而体重和结肠长度恢复。Cur治疗后，脾脏和PPs中的CD8⁺CD11c⁺细胞减少，主要组织相容性复合体II、CD205、CD40、CD40L和细胞间黏附分子-1的表达受到抑制。与未治疗的结肠炎小鼠相比，IL-10、IFN-γ和TGF-β1水平升高。
结论
Cur可有效治疗实验性结肠炎，这是通过抑制CD8⁺CD11c⁺细胞实现的。
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