果蝇雄性减数分裂
Drosophila Male Meiosis.
作者信息
Bonaccorsi Silvia, Gatti Maurizio
机构信息
Department of Biology and Biotechnology "Charles Darwin", Sapienza, University of Rome, P.le. A Moro 5, 00185, Rome, Italy.
Institute of Molecular Biology and Pathology (IBPM) of CNR, Rome, Italy.
出版信息
Methods Mol Biol. 2017;1471:277-288. doi: 10.1007/978-1-4939-6340-9_16.
In Drosophila males, there is no synaptonemal complex and recombination does not occur. Thus, Drosophila male meiosis is a good model system for the analysis of achiasmate chromosome segregation. In addition, due to their large size, the meiotic spindles of Drosophila males are an excellent system for mutational dissection of the mechanisms of spindle assembly. Here, we describe the main techniques for visualization of live Drosophila testes and for preparation of fixed meiotic chromosomes and spindles.
在果蝇雄性个体中，不存在联会复合体，且不会发生重组。因此，果蝇雄性减数分裂是分析无交叉染色体分离的良好模型系统。此外，由于果蝇雄性减数分裂纺锤体体积较大，它是用于对纺锤体组装机制进行突变剖析的绝佳系统。在此，我们描述了用于观察活果蝇睾丸以及制备固定的减数分裂染色体和纺锤体的主要技术。
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