Huang Kai, Sun Jia, Yang Chao, Wang Yunfei, Zhou Bingcong, Kang Chunsheng, Han Lei, Wang Qixue
Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China.
Laboratory of Neuro-Oncology, Tianjin Neurological Institute, Tianjin 300052, P.R. China.
Int J Oncol. 2017 Apr;50(4):1271-1278. doi: 10.3892/ijo.2017.3901. Epub 2017 Mar 7.
HOTAIR is a tumor promoting long non-coding RNA (lncRNA) with roles in multiple cancers. However, the role of HOTAIR in glioma has not been well charaterized. Genes that positively correlated with HOTAIR were identified from the Chinese Glioma Genome Atlas and constructed into an interacting network. In total, 18 genes with P-values <0.01 were further extracted and constructed into a subnetwork. Real-time PCR, western blot and immunofluorescence analyses were employed to examine the expression of the genes after HOTAIR overexpression or knockdown. Intracranial glioblastoma multiform (GBM) models were used to test the potential of HOTAIR as a glioma therapy target. It was discovered that the 18 genes that most significantly correlated with HOTAIR expression formed a cell cycle-related mRNA network, which is positively regulated by HOTAIR. Furthermore, HOTAIR knockdown inhibited mouse intracranial GBM model formation. HOTAIR positively regulates a cell cycle-related mRNA network in glioma, and could be a potential therapeutic target for treating glioma.
HOTAIR是一种具有促进肿瘤作用的长链非编码RNA（lncRNA），在多种癌症中发挥作用。然而，HOTAIR在胶质瘤中的作用尚未得到充分表征。从中国胶质瘤基因组图谱中鉴定出与HOTAIR呈正相关的基因，并构建成一个相互作用网络。总共进一步提取了18个P值<0.01的基因，并构建成一个子网。采用实时PCR、蛋白质印迹和免疫荧光分析来检测HOTAIR过表达或敲低后这些基因的表达。使用颅内多形性胶质母细胞瘤（GBM）模型来测试HOTAIR作为胶质瘤治疗靶点的潜力。研究发现，与HOTAIR表达最显著相关的18个基因形成了一个细胞周期相关的mRNA网络，该网络受HOTAIR的正向调控。此外，HOTAIR敲低抑制了小鼠颅内GBM模型的形成。HOTAIR在胶质瘤中正向调控一个细胞周期相关的mRNA网络，可能是治疗胶质瘤的潜在治疗靶点。
