School of Chemical Engineering and Technology, Hebei University of Technology , Guangrong Dao No.8, Hongqiao District, Tianjin 300130, China.
ACS Appl Mater Interfaces. 2017 Apr 19;9(15):13554-13563. doi: 10.1021/acsami.7b02679. Epub 2017 Apr 10.
Glutathione (GSH) as a biothiol is an essential peptide related to various diseases. Although multiple strategies for biothiols detection have been developed, there is increasing demand for sensors that can differentiate GSH from cysteine (Cys) and homocysteine (Hcy), owing to the similar structures and thiol groups in these amino acids. Herein, we report a novel Eu/LAPONITE (Lap)-based organic/inorganic hybrid material for selective detection of GSH via an "off-on" process. The fluorescence of Eu(DPA)@Lap-Tris can be quenched by Cu through photoinduced electron transfer (PET). The addition of GSH into the Eu(DPA)@Lap-Tris/Cu system induces the removal of Cu from Eu(DPA)@Lap-Tris and blocks PET, resulting in the recovery of fluorescence. This proposed assay demonstrates higher selectivity toward GSH than Cys and Hcy, and showed a detection limit of 162 nM within a linear range of 0.5-30 μM. Unlike other GSH selective sensors, this platform could be formed into a hydrogel while its sensitivity was maintained. The sensitive response to GSH in serum samples makes this platform an efficient tool for biological applications because of its ease of preparation, high selectivity, good biocompatibility, and low toxicity.
谷胱甘肽（GSH）作为一种生物硫醇，与各种疾病有关。尽管已经开发出多种生物硫醇检测策略，但由于这些氨基酸的结构和巯基相似，因此需要能够区分 GSH、半胱氨酸（Cys）和同型半胱氨酸（Hcy）的传感器。在此，我们报告了一种新型的基于 Eu/LAPONITE（Lap）的有机/无机杂化材料，通过“关-开”过程用于选择性检测 GSH。Eu(DPA)@Lap-Tris 的荧光可以通过光诱导电子转移（PET）被 Cu 猝灭。将 GSH 加入 Eu(DPA)@Lap-Tris/Cu 体系中会导致 Cu 从 Eu(DPA)@Lap-Tris 中去除并阻断 PET，从而恢复荧光。与其他 GSH 选择性传感器相比，该测定法对 GSH 的选择性更高，在 0.5-30 μM 的线性范围内检测限为 162 nM。与其他 GSH 选择性传感器不同的是，该平台可以形成水凝胶，同时保持其灵敏度。该平台对血清样品中 GSH 的灵敏响应使其成为生物应用的有效工具，因为它易于制备、高选择性、良好的生物相容性和低毒性。
