Marques André M, Velozo Leosvaldo S, Carvalho Michelle A, Serdeiro Michele T, Honório Nildimar A, Kaplan Maria Auxiliadora C, Maleck Marise
Walter Mors Institute for Research on Natural Products (IPPN), Health Sciences Center, Universidade Federal do Rio de Janeiro, Brazil.
Vector Insect Laboratory, Universidade Severino Sombra, Av. Expedicionário Oswaldo de Almeida Ramos, Rio de Janeiro, Brazil.
J Vector Borne Dis. 2017 Jan-Mar;54(1):61-68.
BACKGROUND & OBJECTIVES: Aedes aegypti mosquito is the principal vector of the viruses responsible for urban yellow fever, dengue, dengue haemorrhagic fever, as well as Zika and chikungunya in Brazil. The present study was aimed to investigate the insecticidal potential of the extract and fractions of Ottonia anisum, along with special metabolites isolated from it, as natural alternatives against larvae (L3) of Ae. aegypti, vector of potentially deadly tropical infections in Brazil.
The plant species O. anisum was collected in March 2015, at Xerιm area, in Rio de Janeiro City, Brazil. Crude extracts and the isolated pure compounds were screened for toxicity against Ae. aegypti larvae (L3). Bioassays were performed on 20 larvae (L3) of Ae. aegypti in triplicate. The samples were dissolved in a mixture of acetone and DMSO at final concentrations of 1-200 μg/ml. The toxicity of the solutions was evaluated towards the growth and development of Ae. aegypti larvae till emergence of adults.
The crude hexane extract showed 100% larval mortality 24 h after treatment at a concentration of 200 μg/ml. The bioassays using 1-butyl-3,4-methylenedioxybenzene revealed 100% mortality among L3 larvae, 24 h afterthe treatment at a concentration of 30 μg/ml, the LC recorded was 1.6 μg/ml. At concentration of 10 μg/ml, the L3 larval mortality recorded was 92%.
INTERPRETATION & CONCLUSION: The metabolite 1-butyl-3,4-methylenedioxybenzene showed potent toxicity against Ae. aegypti larvae. This arylbutanoid agent could be used as a natural alternative adjuvant pesticide, in new compositions that would be environmentally safer.
埃及伊蚊是导致城市黄热病、登革热、登革出血热以及巴西寨卡病毒病和基孔肯雅热的病毒的主要传播媒介。本研究旨在调查茴芹提取物及其馏分以及从中分离出的特殊代谢产物作为对抗巴西潜在致命热带感染媒介埃及伊蚊幼虫（L3）的天然替代品的杀虫潜力。
2015年3月在巴西里约热内卢市的Xerιm地区采集了茴芹这种植物。对粗提物和分离出的纯化合物进行了抗埃及伊蚊幼虫（L3）毒性筛选。对20只埃及伊蚊幼虫（L3）进行生物测定，重复三次。将样品溶解在丙酮和二甲基亚砜的混合物中，终浓度为1 - 200μg/ml。评估溶液对埃及伊蚊幼虫生长发育直至成虫羽化的毒性。
粗己烷提取物在浓度为200μg/ml处理24小时后显示出100%的幼虫死亡率。使用1 - 丁基 - 3, ,4 - 亚甲二氧基苯进行的生物测定显示，在浓度为30μg/ml处理24小时后，L3幼虫死亡率为100%，记录的LC为1.6μg/ml。在浓度为10μg/ml时，记录的L3幼虫死亡率为92%。
代谢产物1 - 丁基 - 3, ,4 - 亚甲二氧基苯对埃及伊蚊幼虫显示出强大的毒性。这种芳基丁烷类药剂可作为天然替代辅助农药，用于环境更安全的新配方中。
