Bhange Khushboo, Chaturvedi Venkatesh, Bhatt Renu
Department of Biotechnology, Guru Ghasidas Vishwavidyalaya (A Central University), Bilaspur 495009, Chhattisgarh, India.
School of Biotechnology, Banaras Hindu University, Varanasi, U.P., India.
Biotechnol Rep (Amst). 2016 Apr 13;10:94-104. doi: 10.1016/j.btre.2016.03.007. eCollection 2016 Jun.
The present study is an attempt to optimize simultaneous production of keratinolytic protease, amylase and biosurfactant from feather meal, potato peel and rape seed cake in a single media by response surface methodology to evaluate their biochemical properties for detergent additive. The optimization was carried out using 20 run, 3 factor and 5-level of central composite design on design expert software which resulted in a 1.2, 0.84 and 2.28 fold increase in protease, amylase and biosurfactant production. The proteolytic activity was found to be optimum at pH 9.0 and 60 °C while optimum amylolytic activity was recorded at pH 6.0 and 70 °C respectively. Both enzymes were found to be stable in the presence of organic solvents, ionic and commercial detergent and oxidizing agents. The biosurfactant was extracted with chloroform and was found to be stable at varying pH and temperature; however a reduction in the activity was observed at temperature higher than 70 °C. The isolated enzymes and biosurfactants may find applications in the effective removal of stains.
本研究旨在通过响应面法优化在单一培养基中从羽毛粉、马铃薯皮和油菜籽饼同时生产角蛋白酶、淀粉酶和生物表面活性剂，以评估它们作为洗涤剂添加剂的生化特性。使用设计专家软件进行了20次运行、3因素和5水平的中心复合设计优化，结果使蛋白酶、淀粉酶和生物表面活性剂的产量分别提高了1.2倍、0.84倍和2.28倍。发现蛋白水解活性在pH 9.0和60℃时最佳，而淀粉酶活性分别在pH 6.0和70℃时记录最佳。发现这两种酶在有机溶剂、离子和商业洗涤剂以及氧化剂存在下均稳定。生物表面活性剂用氯仿提取，发现在不同的pH和温度下稳定；然而，在高于70℃的温度下观察到活性降低。分离出的酶和生物表面活性剂可能在有效去除污渍方面找到应用。
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