甲状腺5'-核苷酸酶的拓扑结构、纯化及特性分析

Topography, purification and characterization of thyroidal 5'-nucleotidase.

Peeters C, de Wolf M, Van Dessel G, Lagrou A, Hilderson H, Dierick W

RUCA-Laboratory for Human Biochemistry, University of Antwerp, Belgium.

Int J Biochem. 1988;20(4):409-19. doi: 10.1016/0020-711x(88)90209-1.

Abstract

Subcellular studies of bovine thyroid indicate that 5'-nucleotidase is predominantly associated with plasma membranes, although a considerable part of this ectoenzyme is also found internalized. 2. The enzyme displaying the features of a glycoprotein has been purified 1400 times by detergent solubilization and two subsequent affinity chromatographic steps. 3. Thyroidal 5'-nucleotidase can be classified as an unspecific metallo-dependent 5'-ribonucleotide phosphohydrolase. The native enzyme exists as a dimer (MW 150 kDalton), composed of two similar or identical subunits.

摘要

对牛甲状腺的亚细胞研究表明，5'-核苷酸酶主要与质膜相关，尽管这种胞外酶的相当一部分也存在于内化状态。2. 这种具有糖蛋白特征的酶已通过去污剂溶解和随后的两步亲和层析纯化了1400倍。3. 甲状腺5'-核苷酸酶可归类为一种非特异性金属依赖性5'-核糖核苷酸磷酸水解酶。天然酶以二聚体形式存在（分子量150千道尔顿），由两个相似或相同的亚基组成。