Jingjing Lin, Wangyue Wang, Qiaoqiao Xu, Jietong Ye
Department of Pharmacy, The First Affiliated Hospital of Wenzhou Medical University, 325000 PR China.
Department of Gastroenterology, The People's Hospital of Lishui, 323000 PR China.
Open Med (Wars). 2016 Mar 11;11(1):31-35. doi: 10.1515/med-2016-0007. eCollection 2016.
Increasing evidence showed that microRNAs (miRNAs) were implicated in the chemical resistance of human cancers. We intended to investigate the role of miR-218 in cisplatin sensitivity of esophageal cancer cells.
Quantitative real-time polymerase chain reaction (qRT-PCR) was carried out to analyze miR-218 expression in human esophageal cancer cell line Eca9706 and a cisplatin-resistant subline (ECa9706-CisR cells). The effects of miR-218 transfection on ECa9706 and ECa9706-CisR cell viability, including cell viability and apoptosis rate were confirmed using MTT assay, or flow cytometry, respectively. qRT-PCR was used to validate survivin as a direct target gene of miR-218 in our system.
We found that miR-218 was significantly decreased in ECa9706-CisR cells compared with parent Eca9706 cells. Overexpression of miR-218 by mimics transfection would enhance cisplatin sensitivity evaluated by cell viability inhibition and apoptosis promotion. We validated here survivin as a direct target of miR-218 in ECa9706 cells, which might contribute to the chemoresistance of esophageal cancer cells to cisplatin.
In summary, our data suggest that miR-218 might represent as a promising sensitizer of cisplatin therapy in clinical esophageal cancer patients.
越来越多的证据表明，微小RNA（miRNA）与人类癌症的化学抗性有关。我们旨在研究miR-218在食管癌细胞顺铂敏感性中的作用。
采用定量实时聚合酶链反应（qRT-PCR）分析miR-218在人食管癌细胞系Eca9706和顺铂耐药亚系（ECa9706-CisR细胞）中的表达。分别使用MTT法或流式细胞术确认miR-218转染对ECa9706和ECa9706-CisR细胞活力（包括细胞活力和凋亡率）的影响。使用qRT-PCR验证survivin是我们系统中miR-218的直接靶基因。
我们发现，与亲本Eca9706细胞相比，ECa9706-CisR细胞中miR-218显著降低。通过模拟转染过表达miR-218可增强顺铂敏感性，这通过细胞活力抑制和凋亡促进来评估。我们在此验证了survivin是ECa9706细胞中miR-218的直接靶标，这可能导致食管癌细胞对顺铂的化疗耐药。
总之，我们的数据表明，miR-218可能是临床食管癌患者顺铂治疗的一种有前景的增敏剂。
