Rong Jiang, Chunhua Ma, Yuan Lv, Ning Mu, Jinduo Li, Bin Wang, Liwei Sun
Department of Intervention, Tianjin Huanhu Hospital, Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases, Tianjin 300060, China.
Open Med (Wars). 2016 Apr 18;11(1):93-96. doi: 10.1515/med-2016-0018. eCollection 2016.
To discuss the application of ARMS method to detect EGFR gene mutation in cerebrospinal fluid of lung adenocarcinoma patients with meningeal metastasis.
5 cases of lung adenocarcinoma were identified with meningeal metastasis that were cleared EGFR gene mutation by gene sequencing method. From each patient 5ml cerebrospinal fluid was obtained by lumbar puncture. ARMS method was used to detect EGFR mutations in cerebrospinal fluid.
5 samples of cerebrospinal fluid were successfully detected by ARMS method, 3 samples found that EGFR gene mutations, the mutations in line with direct sequencing method.
ARMS method can be used to detect EGFR gene mutations of cerebrospinal fluid samples in lung adenocarcinoma with meningeal metastasis. But cerebrospinal fluid specimens from histological specimens, blood samples need to be confirmed by further comparative study whether there is advantage.
探讨扩增阻滞突变系统（ARMS）法检测肺腺癌脑膜转移患者脑脊液中表皮生长因子受体（EGFR）基因突变的应用。
选取5例经基因测序法证实存在EGFR基因突变的肺腺癌脑膜转移患者。通过腰椎穿刺从每位患者获取5ml脑脊液。采用ARMS法检测脑脊液中的EGFR突变。
采用ARMS法成功检测5份脑脊液样本，3份样本检测到EGFR基因突变，且突变结果与直接测序法相符。
ARMS法可用于检测肺腺癌脑膜转移患者脑脊液样本中的EGFR基因突变。但脑脊液标本与组织标本、血液标本相比是否具有优势，尚需进一步对比研究证实。
