Yu Xiaohui, Chen Guoping, Guo Xuhu, Lu Yu, Zhang Jianling, Hu Jingtao, Tian Shibing, Hu Zongli
Laboratory of Molecular Biology of Tomato, Room 515, Bioengineering College, Chongqing University, Campus B, 174 Shapingba Main Street, 400044, Chongqing, People's Republic of China.
The Institute of Vegetable Research, Chongqing Academy of Agricultural Sciences, 401329, Chongqing, People's Republic of China.
Plant Cell Rep. 2017 Jun;36(6):959-969. doi: 10.1007/s00299-017-2129-9. Epub 2017 Mar 28.
Silencing SlAGL6 in tomato leads to fused sepal and green petal by influencing the expression of A-, B-class genes. AGAMOUS-LIKE6 (AGL6) lineage is an important clade MADS-box transcription factor and plays essential roles in various developmental programs especially in flower meristem and floral organ development. Here, we isolated a tomato AGL6 lineage gene SlAGL6 and successfully obtained several RNA interference (RNAi) lines. Silencing SlAGL6 led to abnormal fused sepals and light green petals with smaller size. The total chlorophyll content in transgenic petals increased and the morphology of epidermis cells altered. Further analysis showed that A-class gene MACROCALYX (MC) participating in sepal development and a NAC-domain gene GOBLET involving in boundary establishment were down-regulated in transgenic lines. In transgenic petals, two chlorophyll synthesis genes, Golden2-like1 (SlGLK1) and Golden2-like2 (SlGLK2), two photosystem-related genes, ribulose bisphosphate carboxylase small chain 3B (SlrbcS3B) and chlorophyll a/b-binding protein 7 (SlCab-7) were induced and three B-class genes TM6, TAP3 and SlGLO1 were repressed. These results suggest that SlAGL6 involves in tomato sepal and petal development.
在番茄中沉默SlAGL6会通过影响A类和B类基因的表达导致萼片融合和花瓣变绿。AGAMOUS-LIKE6（AGL6）谱系是一个重要的MADS-box转录因子分支，在各种发育程序中发挥着重要作用，特别是在花分生组织和花器官发育中。在此，我们分离出一个番茄AGL6谱系基因SlAGL6，并成功获得了多个RNA干扰（RNAi）株系。沉默SlAGL6导致萼片异常融合以及花瓣呈浅绿色且尺寸变小。转基因花瓣中的总叶绿素含量增加，表皮细胞形态发生改变。进一步分析表明，参与萼片发育的A类基因MACROCALYX（MC）和参与边界建立的NAC结构域基因GOBLET在转基因株系中表达下调。在转基因花瓣中，两个叶绿素合成基因Golden2-like1（SlGLK1）和Golden2-like2（SlGLK2）、两个光系统相关基因核酮糖二磷酸羧化酶小链3B（SlrbcS3B）和叶绿素a/b结合蛋白7（SlCab-7）被诱导表达，而三个B类基因TM6、TAP3和SlGLO1被抑制。这些结果表明SlAGL6参与番茄萼片和花瓣的发育。
