Cheewachaiwit S, Warin N, Phuangrat B, Rukpratanporn S, Gajanandana O, Balatero C H, Chatchawankanphanich O
Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140, Thailand.
Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE), Bangkok, 10900, Thailand.
Arch Virol. 2017 Jul;162(7):2083-2090. doi: 10.1007/s00705-017-3332-2. Epub 2017 Mar 28.
Overall, 244 samples of cucurbit crops with yellowing symptoms and selected weed species, from 15 provinces in Thailand, were screened by RT-PCR using primers Polero-CP-F and Polero-CP-R. A total of 160 samples (~66%) were infected by poleroviruses. Analysis of a 1.4 kb region covering the 3' RNA-dependent RNA polymerase (RdRp) gene, the intergenic non-coding region (iNCR), and the coat protein (CP), showed that four poleroviruses, namely, cucurbit aphid-borne yellows virus (CABYV), luffa aphid-borne yellows virus (LABYV), melon aphid-borne yellows virus (MABYV) and suakwa aphid-borne yellows virus (SABYV) were associated with the yellowing symptoms in cucurbit crops. Further analyses indicated presence of putative recombinant viruses referred to as CABYV-R and SABYV-R. CABYV-R was derived from the recombination between MABYV and the common strain of CABYV (CABYV-C). SABYV-R was derived from the recombination of MABYV and SABYV.
总体而言，使用引物Polero-CP-F和Polero-CP-R，通过RT-PCR对来自泰国15个省份的244份有黄化症状的葫芦科作物样本和选定的杂草物种进行了筛查。共有160份样本（约66%）感染了粉虱传双生病毒。对一个覆盖3' RNA依赖性RNA聚合酶（RdRp）基因、基因间非编码区（iNCR）和外壳蛋白（CP）的1.4 kb区域的分析表明，四种粉虱传双生病毒，即葫芦蚜传黄化病毒（CABYV）、丝瓜蚜传黄化病毒（LABYV）、甜瓜蚜传黄化病毒（MABYV）和瓠瓜蚜传黄化病毒（SABYV）与葫芦科作物的黄化症状有关。进一步分析表明存在被称为CABYV-R和SABYV-R的推定重组病毒。CABYV-R源自MABYV和CABYV常见株系（CABYV-C）之间的重组。SABYV-R源自MABYV和SABYV的重组。
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