Shang Qiao-xia, Xiang Hai-ying, Han Cheng-gui, Li Da-wei, Yu Jia-lin
Department of Plant Pathology, State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, China.
Virus Res. 2009 Nov;145(2):341-6. doi: 10.1016/j.virusres.2009.07.017. Epub 2009 Aug 5.
Cucurbit aphid-borne yellows virus (CABYV) and Melon aphid-borne yellows virus (MABYV) have been found to be associated with cucurbit yellowing disease in China. Our report identifies for the first time a third distinct polerovirus, tentatively named Suakwa aphid-borne yellows virus (SABYV), infecting Suakwa vegetable sponge. To better understand the distribution and molecular diversity of these three poleroviruses infecting cucurbits, a total of 214 cucurbitaceous crop samples were collected from 25 provinces in China, and were investigated by RT-PCR and sequencing. Of these, 108 samples tested positive for CABYV, while 40 samples from five provinces were positive for MABYV, and SABYV was detected in only 4 samples which were collected in the southern part of China. Forty-one PCR-amplified fragments containing a portion of the RdRp gene, intergenic NCR and CP gene were cloned and sequenced. Sequence comparisons showed that CABYV isolates shared 78.0-79.2% nucleotide sequence identity with MABYV isolates, and 69.7-70.8% with SABYV. Sequence identity between MABYV and SABYV was 73.3-76.5%. In contrast, the nucleotide identities within each species were 93.2-98.7% (CABYV), 98.1-99.9% (MABYV), and 96.1-98.6% (SABYV). Phylogenetic analyses revealed that the polerovirus isolates fit into three distinct groups, corresponding to the three species. The CABYV group could be further divided into two subgroups: the Asia subgroup and the Mediterranean subgroup, based on CP gene and partial RdRp gene sequences. Recombination analysis suggested that MABYV may be a recombinant virus.
在中国，已发现葫芦科蚜传黄化病毒（CABYV）和甜瓜蚜传黄化病毒（MABYV）与葫芦科植物黄化病有关。我们的报告首次鉴定出第三种不同的马铃薯Y病毒属病毒，暂命名为丝瓜蚜传黄化病毒（SABYV），它可感染丝瓜。为了更好地了解这三种感染葫芦科植物的马铃薯Y病毒属病毒的分布和分子多样性，从中国25个省份共采集了214份葫芦科作物样本，并通过逆转录聚合酶链反应（RT-PCR）和测序进行研究。其中，108份样本检测出CABYV呈阳性，而来自五个省份的40份样本MABYV呈阳性，仅在中国南部采集的4份样本中检测到SABYV。对41个包含部分RNA依赖的RNA聚合酶（RdRp）基因、基因间隔区非编码区（NCR）和外壳蛋白（CP）基因的聚合酶链反应（PCR）扩增片段进行了克隆和测序。序列比较表明，CABYV分离株与MABYV分离株的核苷酸序列同一性为78.0 - 79.2%，与SABYV的为69.7 - 70.8%。MABYV与SABYV之间的序列同一性为73.3 - 76.5%。相比之下，每个种内的核苷酸同一性分别为93.2 - 98.7%（CABYV）、98.1 - 99.9%（MABYV）和96.1 - 98.6%（SABYV）。系统发育分析表明，马铃薯Y病毒属病毒分离株分为三个不同的组，对应于这三个种。基于CP基因和部分RdRp基因序列，CABYV组可进一步分为两个亚组：亚洲亚组和地中海亚组。重组分析表明，MABYV可能是一种重组病毒。
