Smith Bradley J, Cassoli Juliana S, Guest Paul C, Martins-de-Souza Daniel
Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Rua Monteiro Lobato, 255, 13083-862, Campinas, SP, Brazil.
UNICAMP's Neurobiology Center, Campinas, SP, Brazil.
Adv Exp Med Biol. 2017;974:229-236. doi: 10.1007/978-3-319-52479-5_19.
A single protein is often capable of binding with many partners, enabling potential effects on either protein, such as modifying its expression or activity. However, due to the complex nature of in vivo systems, it is often difficult to perform nontargeted assays with a protein of interest. Methods in discovery proteomics must be used to find potential interactors to pave the way for additional, more focused studies. This protocol describes the biological steps needed to create an interactome focused on a single protein target through co-immunoprecipitation.
单一蛋白质通常能够与许多伙伴结合，从而对任何一种蛋白质产生潜在影响，比如改变其表达或活性。然而，由于体内系统的复杂性，针对感兴趣的蛋白质进行非靶向分析往往很困难。必须使用发现蛋白质组学中的方法来寻找潜在的相互作用分子，为进一步更有针对性的研究铺平道路。本方案描述了通过免疫共沉淀创建聚焦于单一蛋白质靶点的相互作用组所需的生物学步骤。
