以皮肤成纤维细胞为精神分裂症模型的蛋白质组学分析

Proteomic Profiling of Skin Fibroblasts as a Model of Schizophrenia.

作者信息

Wang Lan, Rahmoune Hassan, Guest Paul C

机构信息

Apitope Technology (Bristol) Ltd, Riverside Court, Beaufort Park, Chepstow, NP16 5UH, UK.

Department of Chemical Engineering and Biotechnology, University of Cambridge, CB2 3RA, Cambridge, UK.

出版信息

Adv Exp Med Biol. 2017;974:305-311. doi: 10.1007/978-3-319-52479-5_29.

DOI:10.1007/978-3-319-52479-5_29

PMID:28353250

Abstract

Since many aspects of schizophrenia are also manifested at the peripheral level in proliferating cell types, this chapter describes the analysis of skin fibroblasts biopsied from living patients. The method focuses on cell culture and sample preparation for characterization of the model. The resulting cell extracts can be analysed by any number of proteomic techniques for identification of biomarker candidates. This approach could help to elucidate the molecular mechanisms associated with the pathophysiology of schizophrenia and provide a useful model for a new target and drug discovery.

摘要

由于精神分裂症的许多方面也在增殖细胞类型的外周水平表现出来，本章描述了对活体患者活检的皮肤成纤维细胞的分析。该方法侧重于细胞培养和用于模型表征的样品制备。所得细胞提取物可通过多种蛋白质组学技术进行分析，以鉴定候选生物标志物。这种方法有助于阐明与精神分裂症病理生理学相关的分子机制，并为新靶点和药物发现提供有用的模型。