稳定同位素标记氨基酸细胞培养质谱分析：从精神疾病角度看

SILAC Mass Spectrometry Profiling: A Psychiatric Disorder Perspective.

作者信息

Duque-Guimarães Daniella, Ong Thomas Prates, de Almeida-Faria Juliana, Guest Paul C, Ozanne Susan E

机构信息

University of Cambridge Metabolic Research Laboratories and MRC Metabolic Diseases Unit, Wellcome Trust-MRC Institute of Metabolic Science, Addenbrooke's Hospital, Cambridge, UK.

Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.

出版信息

Adv Exp Med Biol. 2017;974:289-298. doi: 10.1007/978-3-319-52479-5_27.

DOI:10.1007/978-3-319-52479-5_27

PMID:28353248

Abstract

Stable isotope labelling by amino acids in cell culture (SILAC) is a technique that allows proteomic profiling of cells. In this chapter we describe a protocol for the identification and quantification of newly synthesised proteins. The methodology can be applied to any cultured cell system with relevance to schizophrenia, affective disorders and autism spectrum conditions including those addressing responses to pharmacological stimuli.

摘要

细胞培养中氨基酸稳定同位素标记（SILAC）是一种可对细胞进行蛋白质组分析的技术。在本章中，我们描述了一种鉴定和定量新合成蛋白质的方法。该方法可应用于任何与精神分裂症、情感障碍和自闭症谱系疾病相关的培养细胞系统，包括那些研究对药理刺激反应的系统。