Pavón Andrés, Beloqui Izaskun, Salcedo Juan M, Martin Angel G
StemTek Therapeutics, Derio, 48160, Spain.
Methods Mol Biol. 2017;1590:191-196. doi: 10.1007/978-1-4939-6921-0_14.
Cryopreservation and storage of culture-expanded mesenchymal stem cells (MSCs) is essential for a biobank to maintain a collection of cell lines for research and clinical use. Optimization of cryopreservation protocols and methods to minimize damage to cells during freezing and thawing is critical to ensure reliable availability of viable cells. Controlling the freezing rate and the use of appropriate cryoprotectant, as well as stable storage temperature, can minimize the negative effects on cell viability. In this chapter, protocols for cryopreserving MSCs are described.
培养扩增后的间充质干细胞（MSC）的冷冻保存对于生物样本库维持用于研究和临床应用的细胞系 collection 至关重要。优化冷冻保存方案和方法以最大程度减少冻融过程中对细胞的损伤，对于确保可靠地获得有活力的细胞至关重要。控制冷冻速率、使用合适的冷冻保护剂以及稳定的储存温度，可以将对细胞活力 的负面影响降至最低。在本章中，将描述冷冻保存 MSC 的方案。
