SV40早期编码区域内的两个结构域参与人成纤维细胞的转化。

Two domains within the early coding region of SV40 involved in the transformation of human fibroblasts.

作者信息

de Ronde A, Sol C J, MacDonald M E, Koot M, ter Schegget J, van Strien A, Wouters E, van der Noordaa J

机构信息

Department of Virology, University of Amsterdam, The Netherlands.

出版信息

Intervirology. 1987;28(4):221-31. doi: 10.1159/000150019.

DOI:10.1159/000150019

PMID:2835330

Abstract

The early gene products of simian virus 40 (SV40) transform human fibroblasts, whereas those of the human papovavirus BK (BKV) do not. The early gene products of both SV40 and BKV transform baby rat kidney cells. SV40-BKV chimerics were constructed which were able to transform baby rat kidney cells. Using the SV40-BKV chimerics, two domains within the SV40 large T protein were identified which are involved in the transformation of human fibroblasts.

摘要

猿猴病毒40（SV40）的早期基因产物可转化人成纤维细胞，而人乳头瘤病毒BK（BKV）的早期基因产物则不能。SV40和BKV的早期基因产物均可转化幼鼠肾细胞。构建了能够转化幼鼠肾细胞的SV40 - BKV嵌合体。利用这些SV40 - BKV嵌合体，确定了SV40大T蛋白中两个参与人成纤维细胞转化的结构域。