Lakshmi M S, Sherbet Gajanan V
Department of Zoology, University of Poona, Poona 7, India.
Zoologisches Institut der Universität, Poppelsdorfer Schloß, 53 Bonn a.Rh.
Wilhelm Roux Arch Entwickl Mech Org. 1964 Jul;155(4):424-428. doi: 10.1007/BF00577650.
Trypan blue was injected (0.001-0.002 mg/egg) into zygotes ofRana. The trypan blue-injected series showed much greater mortality than the controls. A probable suppression of gastrulation was seen. 29.7% of the dye-injected and 50.3% of the control embryos developed beyond stage 14. Abnormalities seemed to appear only after neurulation began. Microcephaly, trunk and tail abnormalities occurred though in a small number. Sections of a few embryos revealed disorganized brain and degeneration of the neural tissue. There were no cases of mesodermalization of the notochord.The results are discussed from the view point that trypan blue alters the physical state of proteins in the cytoplasm of the egg.
将台盼蓝（0.001 - 0.002毫克/卵）注射到蛙的受精卵中。注射台盼蓝的实验组死亡率远高于对照组。观察到原肠胚形成可能受到抑制。注射染料的胚胎中有29.7%、对照组胚胎中有50.3%发育到了14期以后。异常似乎仅在神经胚形成开始后出现。出现了小头畸形、躯干和尾部异常，不过数量较少。少数胚胎的切片显示大脑结构紊乱和神经组织退化。没有脊索中胚层化的情况。从台盼蓝改变卵细胞质中蛋白质物理状态的角度对结果进行了讨论。
