Xiao Ying, Ouyang Chenjie, Huang Weiguo, Tang Yunlian, Fu Weiting, Cheng Ailan
Cancer Research Institute, University of South China, Hengyang, China.
Key Laboratory of Tumor Cellular & Molecular Pathology (University of South China), College of Hunan Province, Hengyang, China.
PLoS One. 2017 Mar 29;12(3):e0174383. doi: 10.1371/journal.pone.0174383. eCollection 2017.
Annexin A1 is a member of a large superfamily of glucocorticoid-regulated, calcium- and phospholipid-binding proteins. Our previous studies have shown that the abnormal expression of Annexin A1 is related to the occurrence and development of nasopharyngeal carcinoma (NPC). To understand the roles of Annexin A1 in the tumorigenesis of NPC, targeted proteomic analysis was performed on Annexin A1-associated proteins from NPC cells. We identified 436 proteins associated with Annexin A1, as well as two Annexin A1-interacted key proteins, S100A9 and Vimentin, which were confirmed by co-immunoprecipitation. Gene function classification revealed that the Annexin A1-associated proteins can be grouped into 21 clusters based on their molecular functions. Protein-protein interaction analysis indicated that Annexin A1 /S100A9/Vimentin interactions may be involved in the invasion and metastasis of NPC because they can form complexes in NPC cells. The down-regulation of Annexin A1 in NPC may lead to the overexpression of S100A9/Vimentin, which may increase the possibility of the invasion ability of NPC cells by adjusting the function of cytoskeleton proteins. Results suggested that the biological functions of Annexin A1 in NPC were diverse, and that Annexin A1 can inhibit the in vitro invasive ability of NPC cells through Annexin A1 /S100A9/Vimentin interaction.
膜联蛋白A1是糖皮质激素调节的、钙和磷脂结合蛋白的大型超家族成员。我们之前的研究表明，膜联蛋白A1的异常表达与鼻咽癌（NPC）的发生发展有关。为了解膜联蛋白A1在NPC肿瘤发生中的作用，对NPC细胞中与膜联蛋白A1相关的蛋白质进行了靶向蛋白质组学分析。我们鉴定出436种与膜联蛋白A1相关的蛋白质，以及两种与膜联蛋白A1相互作用的关键蛋白S100A9和波形蛋白，这两种蛋白通过免疫共沉淀得到了证实。基因功能分类显示，与膜联蛋白A1相关的蛋白质可根据其分子功能分为21个簇。蛋白质-蛋白质相互作用分析表明，膜联蛋白A1/S100A9/波形蛋白的相互作用可能参与NPC的侵袭和转移，因为它们可以在NPC细胞中形成复合物。NPC中膜联蛋白A1的下调可能导致S100A9/波形蛋白的过表达，这可能通过调节细胞骨架蛋白的功能增加NPC细胞侵袭能力的可能性。结果表明，膜联蛋白A1在NPC中的生物学功能是多样的，并且膜联蛋白A1可以通过膜联蛋白A1/S100A9/波形蛋白的相互作用抑制NPC细胞的体外侵袭能力。
