来自海洋细菌噬纤维菌属嗜藻亚种的O-特异性多糖的结构
Structure of the O-specific polysaccharide from a marine bacterium Cellulophaga algicola.
作者信息
Tomshich Svetlana V, Kokoulin Maxim S, Kalinovsky Anatoliy I, Nedashkovskaya Ol'ga I, Komandrova Nadezhda A
机构信息
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 690022 Vladivostok, Russia.
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 690022 Vladivostok, Russia.
出版信息
Carbohydr Res. 2017 Apr 18;443-444:68-72. doi: 10.1016/j.carres.2017.03.017. Epub 2017 Mar 22.
The O-polysaccharide was isolated from the lipopolysaccharide of Cellulophaga algicola and studied by chemical analyses along with H and C NMR spectroscopy, including 2D H, H COSY, TOCSY, ROESY, Н, С HSQC, HMBC experiments. It was found that the polysaccharide is built up of branched pentasaccharide repeating units, containing D-mannose (Man), D-glucuronic acid (GlcA), N-acetyl-D-glucosamine (GlcNAc), two L-rhamnose (Rha) residues and O-acetyl groups in a non-stoichiometric amount and has the following structure.
从嗜藻纤维杆菌的脂多糖中分离出O-多糖，并通过化学分析以及氢和碳核磁共振光谱进行研究，包括二维氢氢化学位移相关谱（2D H,H COSY）、全相关谱（TOCSY）、旋转坐标系核Overhauser效应谱（ROESY）、氢碳异核单量子相干谱（H,C HSQC）、异核多键相关谱（HMBC）实验。结果发现，该多糖由分支的五糖重复单元组成，包含D-甘露糖（Man）、D-葡萄糖醛酸（GlcA）、N-乙酰-D-葡萄糖胺（GlcNAc）、两个L-鼠李糖（Rha）残基以及非化学计量的O-乙酰基，其结构如下。
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