Wang L P, Hu J L, Zhao Y, Zhang L, Niu Q
School of Public Health, Shanxi Medical University, Taiyuan 030001, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2017 Feb 20;35(2):81-84. doi: 10.3760/cma.j.issn.1001-9391.2017.02.001.
To investigate the influence of aluminum on microRNA29 (miR29) subtypes miR29a, miR29a*, miR29b1, miR29b2, miR29c1, and miR29c2 and β-site amyloid precursor protein cleaving enzyme 1 (BACE1) in the brain of rats. A total of 40 Sprague-Dawley rats were randomly divided into control group and 15, 30, and 45 μmol/kg groups according to the body weight, with 10 rats in each group. The rats were exposed to aluminum (at a dose of 0.1 ml/100 g body weight) by intraperitoneal injection for 8 weeks. The rats in control group were given 0.9% normal saline, and those in exposure groups were given aluminum-maltolate (equivalent volumesof maltolate and aluminum solution were mixed before exposure) . The cerebral cortex and hippocampus were isolated after exposure ended; Western blotting was used to measure the change in BACE1 expression, and real-time reverse transcription polymerase chain reaction was used to measure the mRNA expression of miR29 subtypes in the cerebral cortex and hippocampus. Compared with the control group, the 45 μmol/kg group had a significant increase in BACE1 expression in the cerebral cortex, and the 30 and 45 μmol/kg groups had significant increases in BACE1 expression in the hippocampus (all <0.05) . Compared with the control group, the 15, 30, and 45 μmol/kg groups had significant reductions in the mRNA expression of miR29a*, miR29b2, miR29c1, and miR29c2 in the cerebral cortex and hippocampus (all <0.01) , and the 45 μmol/kg group had significant reductions in the mRNA expression of miR29a and miR29b1 in the cerebral cortex and hippocampus (all <0.05) . The results of correlation analysis showed that there was no correlation between the mRNA expression of miR29a*, miR29b2, miR29c1, and miR29c2 and BACE1 expression in the cerebral cortex and hippocampus (all >0.05) , while the mRNA expression of miR29a and miR29b1 was negatively correlated with BACE1 expression (cerebral cortex: =-0.987 and -0.981, <0.05; hippocampus: =-0.992 and -0.991, <0.05) . Aluminum can reduce the expression of miR29 subtypes and increase BACE1 expression in the brain, and the expression of miR29a and miR29b1 is negatively correlated with BACE1 expression.
研究铝对大鼠脑中微小RNA29（miR29）亚型miR29a、miR29a*、miR29b1、miR29b2、miR29c1和miR29c2以及β-位点淀粉样前体蛋白裂解酶1（BACE1）的影响。将40只Sprague-Dawley大鼠按体重随机分为对照组以及15、30和45 μmol/kg组，每组10只。通过腹腔注射给予大鼠铝（剂量为0.1 ml/100 g体重），持续8周。对照组大鼠给予0.9%生理盐水，暴露组大鼠给予苹果酸铝（暴露前将等量体积的苹果酸和铝溶液混合）。暴露结束后分离大脑皮层和海马；采用蛋白质印迹法检测BACE1表达变化，采用实时逆转录聚合酶链反应检测大脑皮层和海马中miR29亚型的mRNA表达。与对照组相比，45 μmol/kg组大脑皮层中BACE1表达显著增加，30和45 μmol/kg组海马中BACE1表达显著增加（均P<0.05）。与对照组相比，15、30和45 μmol/kg组大脑皮层和海马中miR29a*、miR29b2、miR29c1和miR29c2的mRNA表达显著降低（均P<0.01），45 μmol/kg组大脑皮层和海马中miR29a和miR29b1的mRNA表达显著降低（均P<0.05）。相关性分析结果显示，大脑皮层和海马中miR29a*、miR29b2、miR29c1和miR29c2的mRNA表达与BACE1表达之间无相关性（均P>0.05），而miR29a和miR29b1的mRNA表达与BACE1表达呈负相关（大脑皮层：r=-0.987和-0.981，P<0.05；海马：r=-0.992和-0.991，P<0.05）。铝可降低大鼠脑中miR29亚型的表达并增加BACE1表达，且miR29a和miR29b1的表达与BACE1表达呈负相关。
