谷氨酸棒杆菌同型丝氨酸激酶-苏氨酸合成酶操纵子的核苷酸序列及精细结构分析

Nucleotide sequence and fine structural analysis of the Corynebacterium glutamicum hom-thrB operon.

作者信息

Peoples O P, Liebl W, Bodis M, Maeng P J, Follettie M T, Archer J A, Sinskey A J

机构信息

Department of Applied Biological Sciences, Massachusetts Institute of Technology, Cambridge 02139.

出版信息

Mol Microbiol. 1988 Jan;2(1):63-72. doi: 10.1111/j.1365-2958.1988.tb00007.x.

DOI:10.1111/j.1365-2958.1988.tb00007.x

PMID:2835591

Abstract

The complete nucleotide sequence of the Corynebacterium glutamicum hom-thrB operon has been determined and the structural genes and promoter region mapped. A polypeptide of Mr 46,136 is encoded by hom and a polypeptide of Mr 32,618 is encoded by thrB. Both predicted protein sequences show amino acid sequence homology to their counterparts in Escherichia coli and Bacillus subtilis. The promoter region has been mapped by S1-nuclease and deletion analysis. Located between -88, RNA start site and -219 (smallest deletion clone with complete activity) are sequence elements similar to those found in E. coli and B. subtilis promoters. Although there are no obvious attenuator-like structures in the 5'-untranslated region, there is a dyad-symmetry element, which may act as an operator.

摘要

已确定谷氨酸棒杆菌的同型丝氨酸脱氢酶-苏氨酸B操纵子的完整核苷酸序列，并绘制了结构基因和启动子区域。同型丝氨酸脱氢酶基因编码一个46,136道尔顿的多肽，苏氨酸B基因编码一个32,618道尔顿的多肽。两个预测的蛋白质序列与其在大肠杆菌和枯草芽孢杆菌中的对应序列显示出氨基酸序列同源性。启动子区域已通过S1核酸酶和缺失分析进行了定位。位于RNA起始位点-88和-219（具有完全活性的最小缺失克隆）之间的序列元件与在大肠杆菌和枯草芽孢杆菌启动子中发现的元件相似。尽管在5'-非翻译区没有明显的衰减子样结构，但有一个二元对称元件，它可能作为一个操纵基因起作用。