Follettie M T, Shin H K, Sinskey A J
Department of Applied Biological Sciences, Massachusetts Institute of Technology, Cambridge 02139.
Mol Microbiol. 1988 Jan;2(1):53-62. doi: 10.1111/j.1365-2958.1988.tb00006.x.
The genes encoding the three terminal enzymes in the threonine biosynthetic pathway, homoserine dehydrogenase (hom), homoserine kinase (thrB) and threonine synthase (thrC) have been isolated from Corynebacterium glutamicum. The C. glutamicum hom and thrB genes were subcloned on a 3.6 kb SalI-generated chromosomal fragment. The C. glutamicum thrC gene was shown not to be linked to the hom-thrB locus. L-methionine represses the cloned homoserine dehydrogenase and homoserine kinase similar to that of the chromosomally encoded hom and thrB gene products. Northern hybridization analysis demonstrates that this repression is mediated at the level of transcription and that hom-thrB represents an operon in C. glutamicum.
编码苏氨酸生物合成途径中三种末端酶的基因，即高丝氨酸脱氢酶（hom）、高丝氨酸激酶（thrB）和苏氨酸合酶（thrC），已从谷氨酸棒杆菌中分离出来。谷氨酸棒杆菌的hom和thrB基因被亚克隆到一个由SalI产生的3.6 kb染色体片段上。已证明谷氨酸棒杆菌的thrC基因与hom-thrB基因座不连锁。L-甲硫氨酸对克隆的高丝氨酸脱氢酶和高丝氨酸激酶的抑制作用，与染色体编码的hom和thrB基因产物相似。Northern杂交分析表明，这种抑制作用是在转录水平介导的，并且hom-thrB在谷氨酸棒杆菌中代表一个操纵子。
Zotero 插件