State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Environ Geochem Health. 2018 Oct;40(5):1877-1886. doi: 10.1007/s10653-017-9948-z. Epub 2017 Mar 29.
Perfluoroalkyl acids (PFAAs) are ubiquitous in the environment. They are prone to accumulate in organisms and have raised public attention in recent decades. Feather samples have been successfully applied as nondestructive indicators for several contaminants. However, a sophisticated analytical method for determining PFAAs in feathers is still lacking. In the present study, a series of conditions, such as the use of the solid-phase extraction cartridge type and extraction/digestion methods, were optimized for the analysis of 13 PFAAs in feathers. According to the spiked recoveries, a weak-anion exchange cartridge was chosen and the methanol was selected as the extraction solvent. In the present study, an optimized pretreatment procedure combined with high-performance liquid chromatography-electrospray ionization-tandem mass spectrometric (HPLC-ESI-MS/MS) method was established for the determination of PFAAs in feathers. The recoveries and method detection limits of the PFAAs ranged from 71 to 120% and 0.16 to 0.54 ng/g, respectively. Finally, 13 PFAAs in four accipiter feather samples from Nam Co Basin, Tibetan Plateau, were analyzed, indicating that PFOS was the predominant PFAA in accipiter feathers, with an average of 4.67 ng/g, followed by the short-chain PFAAs, PFBS and PFBA, with averages of 1.91 and 1.39 ng/g, respectively. These results partly indicated the current situation of PFAA pollution in the Nam Co Basin, especially the existence of short-chain PFAAs in this region.
全氟烷基酸（PFAAs）在环境中无处不在。它们易于在生物体内积累，近几十年来引起了公众的关注。羽毛样本已成功用作多种污染物的非破坏性指标。然而，用于测定羽毛中 PFAAs 的复杂分析方法仍然缺乏。在本研究中，优化了一系列条件，例如固相萃取柱类型和提取/消解方法，用于分析羽毛中的 13 种 PFAAs。根据加标回收率，选择了弱阴离子交换柱，甲醇作为提取溶剂。在本研究中，建立了一种优化的预处理程序，结合高效液相色谱-电喷雾电离串联质谱（HPLC-ESI-MS/MS）方法，用于测定羽毛中的 PFAAs。PFAAs 的回收率和方法检测限范围分别为 71%至 120%和 0.16 至 0.54 ng/g。最后，分析了来自青藏高原纳木错流域的 4 只雀鹰羽毛样本中的 13 种 PFAAs，结果表明 PFOS 是雀鹰羽毛中主要的 PFAAs，平均含量为 4.67 ng/g，其次是短链 PFAAs，PFBS 和 PFBA，平均含量分别为 1.91 和 1.39 ng/g。这些结果部分表明了纳木错流域 PFAAs 污染的现状，特别是该地区存在短链 PFAAs。
