• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

转导素对视网膜视杆细胞环鸟苷酸磷酸二酯酶的激活作用：磷酸二酯酶抑制剂与转导素α亚基形成的复合物的特性

Activation of retinal rod cyclic GMP-phosphodiesterase by transducin: characterization of the complex formed by phosphodiesterase inhibitor and transducin alpha-subunit.

作者信息

Deterre P, Bigay J, Robert M, Pfister C, Kühn H, Chabre M

机构信息

Laboratoire de Biophysique Moléculaire et Cellulaire (Unité Associée 520 du CNRS), DRF-CEN Grenoble, France.

出版信息

Proteins. 1986 Oct;1(2):188-93. doi: 10.1002/prot.340010210.

DOI:10.1002/prot.340010210
PMID:2835763
Abstract

The GTP-binding subunit of transducin (T alpha) activates the cGMP phosphodiesterase (PDE) of bovine retinal rods by relieving the constraint imposed by the inhibitory subunit PDE gamma. We have isolated and characterized the complex T alpha.GTP gamma S-PDE gamma formed when T alpha is activated by the nonhydrolyzable analog GTP gamma S. Sedimentation and light-scattering techniques demonstrate that, in contrast to free T alpha.GTP gamma S, which is soluble, the T alpha.GTP gamma S-PDE gamma complex, as well as T alpha.GTP-PDE gamma, is membrane bound at cytosolic ionic strength. It is eluted from the membrane at low ionic strength as a monomeric and 1:1 stoichiometric complex. The relative affinities of PDE gamma for PDE alpha beta and for T alpha.GTP are discussed.

摘要

转导素的GTP结合亚基（Tα）通过解除抑制性亚基PDEγ所施加的限制，激活牛视网膜杆细胞的cGMP磷酸二酯酶（PDE）。我们已经分离并鉴定了Tα被不可水解类似物GTPγS激活时形成的复合物Tα.GTPγS-PDEγ。沉降和光散射技术表明，与可溶的游离Tα.GTPγS不同，Tα.GTPγS-PDEγ复合物以及Tα.GTP-PDEγ在胞质离子强度下与膜结合。在低离子强度下，它以单体和1:1化学计量比的复合物形式从膜上洗脱下来。讨论了PDEγ对PDEαβ和Tα.GTP的相对亲和力。

相似文献

1
Activation of retinal rod cyclic GMP-phosphodiesterase by transducin: characterization of the complex formed by phosphodiesterase inhibitor and transducin alpha-subunit.转导素对视网膜视杆细胞环鸟苷酸磷酸二酯酶的激活作用：磷酸二酯酶抑制剂与转导素α亚基形成的复合物的特性
Proteins. 1986 Oct;1(2):188-93. doi: 10.1002/prot.340010210.
2
Reciprocal control of retinal rod cyclic GMP phosphodiesterase by its gamma subunit and transducin.视网膜视杆细胞环鸟苷酸磷酸二酯酶受其γ亚基和转导素的相互调控。
Proteins. 1986 Sep;1(1):90-9. doi: 10.1002/prot.340010114.
3
[Oligonucleotide-directed mutagenesis of inhibitory gamma-subunits of cGMP phosphodiesterase from bovine outer rod segments. A new hypothesis on mechanisms for inhibiting catalytic subunits by gamma-subunits and activation of a holoenzyme by transducin].[牛视杆外段cGMP磷酸二酯酶抑制性γ亚基的寡核苷酸定向诱变。关于γ亚基抑制催化亚基及转导素激活全酶机制的新假说]
Bioorg Khim. 1994 Aug-Sep;20(8-9):821-32.
4
The regulation of the cyclic GMP phosphodiesterase by the GDP-bound form of the alpha subunit of transducin.转导蛋白α亚基的GDP结合形式对环鸟苷酸磷酸二酯酶的调节。
J Biol Chem. 1989 Mar 15;264(8):4490-7.
5
cGMP phosphodiesterase of retinal rods is regulated by two inhibitory subunits.视网膜视杆细胞的环磷酸鸟苷磷酸二酯酶受两个抑制亚基调控。
Proc Natl Acad Sci U S A. 1988 Apr;85(8):2424-8. doi: 10.1073/pnas.85.8.2424.
6
Interaction between the retinal cyclic GMP phosphodiesterase inhibitor and transducin. Kinetics and affinity studies.视网膜环鸟苷酸磷酸二酯酶抑制剂与转导素之间的相互作用。动力学和亲和力研究。
Biochemistry. 1993 Aug 24;32(33):8636-45. doi: 10.1021/bi00084a035.
7
Suppression of GTP/T alpha-dependent activation of cGMP phosphodiesterase by ADP-ribosylation by its gamma subunit in amphibian rod photoreceptor membranes.在两栖类动物视杆光感受器膜中，其γ亚基通过ADP-核糖基化作用对GTP/Tα依赖性的cGMP磷酸二酯酶激活的抑制作用。
Biochemistry. 1999 Jun 15;38(24):7755-63. doi: 10.1021/bi990106a.
8
Membrane stimulation of cGMP phosphodiesterase activation by transducin: comparison of phospholipid bilayers to rod outer segment membranes.转导蛋白对环鸟苷酸磷酸二酯酶激活的膜刺激作用：磷脂双层膜与视杆细胞外段膜的比较
Biochemistry. 1992 Oct 6;31(39):9502-12. doi: 10.1021/bi00154a024.
9
Resonance energy transfer as a direct monitor of GTP-binding protein-effector interactions: activated alpha-transducin binding to the cGMP phosphodiesterase in the bovine phototransduction cascade.共振能量转移作为GTP结合蛋白-效应器相互作用的直接监测手段：活化的α-转导蛋白与牛视觉转导级联反应中的cGMP磷酸二酯酶结合。
Biochemistry. 1991 Jul 23;30(29):7112-8. doi: 10.1021/bi00243a011.
10
Activated cGMP phosphodiesterase of retinal rods. A complex with transducin alpha subunit.视网膜视杆细胞中活化的环鸟苷酸磷酸二酯酶。一种与转导蛋白α亚基的复合物。
J Biol Chem. 1992 Apr 5;267(10):6620-7.

引用本文的文献

1
Mechanistic insights into the role of prenyl-binding protein PrBP/δ in membrane dissociation of phosphodiesterase 6.对异戊二烯结合蛋白PrBP/δ在磷酸二酯酶6膜解离中作用的机制性见解。
Nat Commun. 2018 Jan 8;9(1):90. doi: 10.1038/s41467-017-02569-y.
2
Functional mapping of interacting regions of the photoreceptor phosphodiesterase (PDE6) γ-subunit with PDE6 catalytic dimer, transducin, and regulator of G-protein signaling9-1 (RGS9-1).光感受器磷酸二酯酶（PDE6）γ亚基与 PDE6 催化二聚体、转导蛋白和 G 蛋白信号转导调节因子 9-1（RGS9-1）相互作用区域的功能图谱。
J Biol Chem. 2012 Jul 27;287(31):26312-20. doi: 10.1074/jbc.M112.377333. Epub 2012 Jun 4.
3
Mechanism for the regulation of mammalian cGMP phosphodiesterase6. 2: isolation and characterization of the transducin-activated form.
哺乳动物 cGMP 磷酸二酯酶 6.2 的调节机制。2：转导蛋白激活形式的分离与鉴定。
Mol Cell Biochem. 2010 Jun;339(1-2):235-51. doi: 10.1007/s11010-010-0404-y. Epub 2010 Feb 23.
4
Mechanism for the regulation of mammalian cGMP phosphodiesterase6. 1: identification of its inhibitory subunit complexes and their roles.哺乳动物环鸟苷酸磷酸二酯酶 6.1 的调节机制：其抑制性亚基复合物的鉴定及其作用。
Mol Cell Biochem. 2010 Jun;339(1-2):215-33. doi: 10.1007/s11010-010-0387-8. Epub 2010 Feb 12.
5
The ordered visual transduction complex of the squid photoreceptor membrane.鱿鱼光感受器膜中有序的视觉转导复合体。
Mol Neurobiol. 1999 Aug;20(1):61-80. doi: 10.1007/BF02741365.
6
Identification of a binding site on retinal transducin alpha for the phosphodiesterase inhibitory gamma subunit.视网膜转导蛋白α上磷酸二酯酶抑制性γ亚基结合位点的鉴定。
Biochem J. 1994 Jan 1;297 ( Pt 1)(Pt 1):87-91. doi: 10.1042/bj2970087.
7
Tryptophan W207 in transducin T alpha is the fluorescence sensor of the G protein activation switch and is involved in the effector binding.转导蛋白Tα中的色氨酸W207是G蛋白激活开关的荧光传感器，并参与效应器结合。
EMBO J. 1993 Nov;12(11):4191-8. doi: 10.1002/j.1460-2075.1993.tb06103.x.
8
cGMP binding sites on photoreceptor phosphodiesterase: role in feedback regulation of visual transduction.光感受器磷酸二酯酶上的cGMP结合位点：在视觉转导反馈调节中的作用
Proc Natl Acad Sci U S A. 1994 May 24;91(11):4845-9. doi: 10.1073/pnas.91.11.4845.
9
Photic modulation of a highly sensitive, near-infrared light-scattering signal recorded from intact retinal photoreceptors.从完整视网膜光感受器记录到的高灵敏度近红外光散射信号的光调制。
Proc Natl Acad Sci U S A. 1988 Aug;85(15):5531-5. doi: 10.1073/pnas.85.15.5531.
10
Light and GTP dependence of transducin solubility in retinal rods. Further analysis by near infra-red light scattering.视网膜视杆细胞中传导素溶解性的光和GTP依赖性。通过近红外光散射进行的进一步分析。
Eur Biophys J. 1988;16(4):207-18. doi: 10.1007/BF00261263.