Westcott J Y, Murphy R C, Stenmark K
Department of Pharmacology, University of Colorado Health Sciences Center, Denver 80262.
Prostaglandins. 1987 Dec;34(6):877-87. doi: 10.1016/0090-6980(87)90068-2.
Recent evidence has shown that a variety of prostaglandins and leukotrienes can be produced in brain tissue after injury in animals. It has also been speculated that increases in brain prostaglandins occur in humans following injury. Ventricular cerebrospinal fluid (CSF) samples have been obtained from children with static lesions (controls) as well as children with acute brain injury and eicosanoids measured by immunologic techniques. Metabolites of prostacyclin (6-keto-PGF1 a) and thromboxane A2 (thromboxane B2) were the major eicosanoids found in CSF, and levels of these compounds were increased 3-10 times in acutely injured patients. Prostaglandin E2 was also found in lower amounts, although in one case its level was very high. Prostaglandin D2 was also present, but in low amounts. No leukotrienes were found in CSF samples that were purified by HPLC prior to immunoassay. Elevated levels of hydroxyeicosatetraenoic acids (HETEs) were observed in those samples stored frozen, but these metabolites were most probably due to autooxidation of arachidonic acid in CSF. Arachidonic acid concentration in CSF was typically found to be in the range of 10-200 ng/ml, but was found to be 5-10 fold higher in one severely injured patient. Thus, elevated free arachidonic acid and various oxygenated metabolites were observed in CSF following brain injury.
最近有证据表明，动物受伤后，脑组织中可产生多种前列腺素和白三烯。也有人推测，人类受伤后，脑内前列腺素会增加。已从患有静态病变的儿童（对照组）以及患有急性脑损伤的儿童中获取脑室脑脊液（CSF）样本，并通过免疫技术测定类花生酸。前列环素（6-酮-PGF1α）和血栓素A2（血栓素B2）的代谢产物是脑脊液中发现的主要类花生酸，在急性受伤患者中，这些化合物的水平增加了3至10倍。还发现了少量的前列腺素E2，尽管在一个病例中其水平非常高。也存在前列腺素D2，但含量很低。在免疫测定前通过HPLC纯化的脑脊液样本中未发现白三烯。在冷冻保存的那些样本中观察到羟基二十碳四烯酸（HETEs）水平升高，但这些代谢产物很可能是由于脑脊液中花生四烯酸的自动氧化所致。脑脊液中花生四烯酸的浓度通常在10至200 ng/ml范围内，但在一名重伤患者中发现其浓度高出五至十倍。因此，脑损伤后在脑脊液中观察到游离花生四烯酸和各种氧化代谢产物升高。
