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金毛狗脊多糖通过促进G1/S期细胞周期转换来刺激体外软骨细胞增殖。

Cibotium barometz polysaccharides stimulate chondrocyte proliferation in vitro by promoting G1/S cell cycle transition.

作者信息

Fu Changlong, Zheng Chunsong, Lin Jie, Ye Jinxia, Mei Yangyang, Pan Caibin, Wu Guangwen, Li Xihai, Ye Hongzhi, Liu Xianxiang

机构信息

Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China.

出版信息

Mol Med Rep. 2017 May;15(5):3027-3034. doi: 10.3892/mmr.2017.6412. Epub 2017 Mar 30.

DOI:10.3892/mmr.2017.6412
PMID:28358416
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5428555/
Abstract

Cibotium barometz polysaccharides (CBPS) are one of the most important bioactive components extracted from the Cibotium barometz plant, which belongs to the Dicksoniaceae family. It has been widely used for the treatment of orthopedic diseases in traditional Chinese medicine. However, the molecular mechanisms behind the therapeutic effects of CBPS remain to be clarified. In the present study, the concentration of CBPS was detected by phenol-vitriol colorimetry. Furthermore, the effects stimulated by CBPS on the viability and G1/S cell cycle transition in primary chondrocytes from Sprague-Dawley rats were investigated. A cell viability assay demonstrated that chondrocyte proliferation may be enhanced by CBPS in a dose‑ and time‑dependent manner. The mechanism underlying the promotion of chondrocyte cell cycle was suggested to involve the stimulation of G1 to S phase transition. To further confirm the results, reverse transcription‑quantitative polymerase chain reaction and western blot analyses were used to detect the expression of mRNA and protein levels of cyclin D1, cyclin‑dependent kinase 4 and retinoblastoma protein. The results suggested that CBPS may stimulate chondrocyte proliferation via promoting G1/S cell cycle transition. Since osteoarthritis is characterized by deficient proliferation in chondrocytes, the present study indicates that CBPS may potentially serve as a novel method for the treatment of osteoarthritis.

摘要

金毛狗脊多糖（CBPS）是从属于蚌壳蕨科的金毛狗脊植物中提取的最重要的生物活性成分之一。在传统中医中，它已被广泛用于治疗骨科疾病。然而，CBPS治疗效果背后的分子机制仍有待阐明。在本研究中，采用苯酚 - 硫酸比色法检测CBPS的浓度。此外，研究了CBPS对Sprague-Dawley大鼠原代软骨细胞活力和G1/S细胞周期转换的影响。细胞活力测定表明，CBPS可能以剂量和时间依赖性方式增强软骨细胞增殖。软骨细胞细胞周期促进的潜在机制被认为涉及刺激从G1期到S期的转变。为了进一步证实结果，采用逆转录定量聚合酶链反应和蛋白质印迹分析来检测细胞周期蛋白D1、细胞周期蛋白依赖性激酶4和视网膜母细胞瘤蛋白的mRNA和蛋白质水平的表达。结果表明，CBPS可能通过促进G1/S细胞周期转换来刺激软骨细胞增殖。由于骨关节炎的特征是软骨细胞增殖不足，本研究表明CBPS可能潜在地作为一种治疗骨关节炎的新方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92cc/5428555/5b08d1ce0331/MMR-15-05-3027-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92cc/5428555/fb017cb8f6d2/MMR-15-05-3027-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92cc/5428555/de631848a7b6/MMR-15-05-3027-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92cc/5428555/3272770b81a6/MMR-15-05-3027-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92cc/5428555/65a010ea8b2d/MMR-15-05-3027-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92cc/5428555/1898a396354f/MMR-15-05-3027-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92cc/5428555/e252485b8c9e/MMR-15-05-3027-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92cc/5428555/5b08d1ce0331/MMR-15-05-3027-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92cc/5428555/fb017cb8f6d2/MMR-15-05-3027-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92cc/5428555/de631848a7b6/MMR-15-05-3027-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92cc/5428555/3272770b81a6/MMR-15-05-3027-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92cc/5428555/65a010ea8b2d/MMR-15-05-3027-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92cc/5428555/1898a396354f/MMR-15-05-3027-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92cc/5428555/e252485b8c9e/MMR-15-05-3027-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92cc/5428555/5b08d1ce0331/MMR-15-05-3027-g06.jpg

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