Prearo Moço Natália, Camargo Batista Renata Aparecida, Fernandes Martin Laura, de Oliveira Leandro Gustavo, Garcia de Lima Parada Cristina Maria, Alarcão Dias-Melicio Luciane, de Assis Golim Marjorie, Guimarães da Silva Márcia
Department of Pathology, São Paulo State University (UNESP), Botucatu, Brazil.
Gynecol Obstet Invest. 2018;83(1):1-8. doi: 10.1159/000468930. Epub 2017 Mar 31.
The inflammatory response in preterm parturition is regulated by the innate immune system. Toll-like receptors (TLR)-2 and TLR-4 are innate immune receptors that recognize the microorganisms most frequently involved in amniotic cavity infections, which are associated with activating the inflammatory response at the maternal-fetal interface during preterm labor. This study aimed to evaluate the expression of TLR-2 and TLR-4 in maternal neutrophils in preterm labor.
A cross-sectional study was conducted in the Obstetrics Care Unit of Botucatu Medical School, UNESP, Brazil. The preterm group was composed of 20 pregnant women who presented preterm labor and preterm delivery. The control group was composed of 20 nonlaboring pregnant women matched to the preterm group by gestational age. Neutrophils were isolated from peripheral blood and TLR expressions were performed by real-time polymerase chain reaction and flow cytometry.
Gene expressions of TLR-2 and TLR-4 in neutrophils from the preterm group were statistically higher than expressions in neutrophils from the matched control group. The percentage of TLR-4+ neutrophils was higher in the preterm group than the matched control group, while the percentage of TLR-2+ neutrophils did not differ between groups.
TLR-4 expression in maternal neutrophils is associated with spontaneous preterm labor.
早产时的炎症反应受先天性免疫系统调节。Toll样受体（TLR）-2和TLR-4是先天性免疫受体，可识别羊膜腔感染中最常见的微生物，这些微生物与早产时母胎界面的炎症反应激活有关。本研究旨在评估早产产妇中性粒细胞中TLR-2和TLR-4的表达。
在巴西圣保罗州立大学博图卡图医学院妇产科护理单元进行了一项横断面研究。早产组由20例早产和早产分娩的孕妇组成。对照组由20例与早产组孕周匹配的未分娩孕妇组成。从外周血中分离中性粒细胞，并通过实时聚合酶链反应和流式细胞术检测TLR表达。
早产组中性粒细胞中TLR-2和TLR-4的基因表达在统计学上高于匹配对照组中性粒细胞中的表达。早产组中TLR-4+中性粒细胞的百分比高于匹配对照组，而TLR-2+中性粒细胞的百分比在两组之间没有差异。
产妇中性粒细胞中TLR-4的表达与自发性早产有关。
