Baĭkov A A, Kasho V N, Avaeva S M
Bioorg Khim. 1987 Dec;13(12):1681-2.
Inorganic pyrophosphatase isolated from Escherichia coli has been proposed as a label in heterogeneous enzyme immunoassays. The enzyme is remarkably stable and insensitive to sodium azide. Enzyme-antibody conjugates were prepared with glutaraldehyde and purified by gel filtration. Enzyme activity was measured by means of a sensitive colour reaction between phosphomolybdate and malachite green. A 5-10-fold increase is sensitivity in terms of absorbance readings was observed compared to peroxidase-based assays. The colour change (yellow/greenish blue) inherent in the use of pyrophosphatase as the labelling agent is highly suitable for visual analysis.
从大肠杆菌中分离出的无机焦磷酸酶已被提议作为非均相酶免疫分析中的一种标记物。该酶非常稳定，对叠氮化钠不敏感。用戊二醛制备酶-抗体结合物，并通过凝胶过滤进行纯化。酶活性通过磷钼酸盐和孔雀石绿之间的灵敏显色反应来测定。与基于过氧化物酶的分析相比，在吸光度读数方面观察到灵敏度提高了5至10倍。使用焦磷酸酶作为标记剂所固有的颜色变化（黄色/蓝绿色）非常适合目视分析。
