Mimura K
Nagasaki University, Faculty of Liberal Arts, Japan.
Brain Res. 1988 Apr 5;445(2):228-33. doi: 10.1016/0006-8993(88)91183-3.
Deprivation of visual experience for a short time during the early period postemergence in the fly influences strongly the development of visual pattern discrimination. Cytochrome oxidase histochemistry was used to examine the effect of visual deprivation on the development of the neuronal network in the optic lobe. Flies in which one compound eye was covered immediately after emergence were raised under normal light and dark conditions (LD). Other flies were raised without covering of the compound eyes under LD or continuous darkness (DD) as controls. At various periods of postemergence flies from the 3 groups were used for histochemistry. The results indicated that the deprivation caused by unilateral eye covering produced a decrease in cytochrome oxidase staining of the covered side of the retina and optic lobe, but not in the central part of the brain. This asymmetry in staining was not seen in flies of 1-5 days postemergence but became evident after the sixth day postemergence. When flies were raised in DD until the 5th day postemergence and then in LD, the development of asymmetry in cytochrome oxidase staining was delayed. These results confirm previous behavioral experiments on visual deprivation. Both suggest that visual deprivation during the early period of postemergence leads to a long-lasting decrease in neuronal activity.
果蝇羽化后早期短时间的视觉经验剥夺会强烈影响视觉模式辨别能力的发展。利用细胞色素氧化酶组织化学方法来检测视觉剥夺对视叶神经元网络发育的影响。将羽化后立即遮盖一只复眼的果蝇置于正常的光照和黑暗条件（LD）下饲养。其他果蝇作为对照，在LD条件下或持续黑暗（DD）条件下饲养，不遮盖复眼。在羽化后的不同时期，取这3组果蝇进行组织化学检测。结果表明，单侧眼遮盖造成的剥夺使视网膜和视叶被遮盖一侧的细胞色素氧化酶染色减少，但大脑中央部分未出现这种情况。这种染色不对称现象在羽化后1 - 5天的果蝇中未观察到，但在羽化后第六天变得明显。当果蝇在DD条件下饲养至羽化后第5天，然后置于LD条件下时，细胞色素氧化酶染色不对称的发育会延迟。这些结果证实了先前关于视觉剥夺的行为实验。两者都表明羽化后早期的视觉剥夺会导致神经元活动长期减少。
