de Vente J, Bol J G, Hudson L, Schipper J, Steinbusch H W
Department of Pharmacology, Faculty of Medicine, Free University, Amsterdam, The Netherlands.
Brain Res. 1988 Apr 19;446(2):387-95. doi: 10.1016/0006-8993(88)90900-6.
Atrial natriuretic factor (ANF)-responding, cyclic guanosine monophosphate (cGMP)-producing cells were visualized in the hippocampus of the rat applying cGMP immunocytochemistry to hippocampal tissue slices incubated in vitro. These cells were found scattered throughout the hippocampus with their highest density in the hilus fascia dentata. Staining with an antibody against glial fibrillary acidic protein showed a completely different pattern, suggesting a non-glial nature of the ANF-responding cells. cGMP accumulation assayed biochemically was observed already at 10 microM ANF and was sensitive to inhibition by Methylene blue. The immunocytochemical data were fully supported by the biochemical measurements of cGMP under these conditions. Our results show for the first time a response to ANF of individual, cGMP-producing cells in the CNS of the rat.
运用环磷酸鸟苷（cGMP）免疫细胞化学技术，对体外培养的大鼠海马组织切片进行处理，从而在大鼠海马中观察到了对心房钠尿肽（ANF）有反应且能产生环磷酸鸟苷（cGMP）的细胞。这些细胞分散于整个海马中，在齿状回门区密度最高。用抗胶质纤维酸性蛋白抗体染色呈现出完全不同的模式，这表明对ANF有反应的细胞并非神经胶质细胞。生化检测显示，在10微摩尔ANF作用下即可观察到cGMP积累，且其对亚甲蓝抑制敏感。在这些条件下，cGMP的生化测量结果完全支持了免疫细胞化学数据。我们的结果首次表明大鼠中枢神经系统中单个产生cGMP的细胞对ANF有反应。
