Rosen A, van der Merwe P A, Davidson J S
Department of Chemical Pathology, University of Cape Town Medical School, South Africa.
Cancer Res. 1988 Jun 15;48(12):3485-9.
The effect of SV40 viral transformation of human fibroblasts on intercellular gap junctional communication (IJC) was investigated using a short-term quantitative assay. IJC was measured using metabolic cooperation in a coculture system of argininosuccinate synthetase- and argininosuccinate lyase-deficient human fibroblasts. These cell lines were transformed with origin-defective adenovirus/SV40 recombinant virions, and IJC was determined both between transformed cells (homologous IJC) and between transformed and untransformed cells (heterologous IJC). At equivalent cell densities, homologous IJC between transformed cells was reduced to 25-55% of the level between untransformed cells. Intermediate levels of IJC (50-70% of normal) were observed in heterologous cocultures of transformed with untransformed cells. Transformed and untransformed cells were equally sensitive to inhibition of IJC by phorbol esters and by glycyrrhetinic acid, and also did not differ in the degree of upregulation of IJC by forskolin. We conclude that SV40 transformation of human fibroblasts leads to a partial impairment of IJC which is additive when both communicating partners are transformed.
利用一种短期定量测定法，研究了猿猴空泡病毒40（SV40）对人成纤维细胞进行病毒转化对细胞间缝隙连接通讯（IJC）的影响。在精氨琥珀酸合成酶和精氨琥珀酸裂解酶缺陷的人成纤维细胞共培养系统中，利用代谢合作来测量IJC。这些细胞系用缺陷型腺病毒/SV40重组病毒粒子进行转化，并且在转化细胞之间（同源IJC）以及转化细胞与未转化细胞之间（异源IJC）测定IJC。在等效细胞密度下，转化细胞之间的同源IJC降至未转化细胞之间水平的25%-55%。在用未转化细胞与转化细胞进行的异源共培养中，观察到IJC处于中等水平（正常水平的50%-70%）。转化细胞和未转化细胞对佛波酯和甘草次酸抑制IJC同样敏感，并且在福司可林对IJC的上调程度方面也没有差异。我们得出结论，人成纤维细胞的SV40转化导致IJC部分受损，当两个通讯伙伴都被转化时，这种受损具有累加性。
