Maggi M, Genazzani A D, Giannini S, Torrisi C, Baldi E, di Tomaso M, Munson P J, Rodbard D, Serio M
Department of Clinical Physiopathology, University of Florence, Italy.
Endocrinology. 1988 Jun;122(6):2970-80. doi: 10.1210/endo-122-6-2970.
In view of the presence of distinct oxytocin (OT) and vasopressin (VP) receptors in the male genital tract (porcine) we have reexamined the receptors for OT and AVP in the classical OT target tissue, female genital tract (rabbit). Neurohypophysial hormone receptors have been investigated in vagina, myometrium, and oviduct using quantitative ligand binding, adenylate cyclase, and contractility studies. Our results clearly indicate the presence of distinct OT and V1 VP receptors in the myometrium, while only the latter was detected in vagina and oviduct. In myometrium, estrogen treatment increases the density of OT and AVP receptors, while progesterone administration inhibits the estrogen effect. At the time of spontaneous delivery a dramatic (17-fold) increase was observed for the OT sites, while the AVP sites were unchanged. AVP receptors in vagina were sensitive to sex steroid administration and were reduced during pregnancy and delivery. Isometric contractility studies suggest that not just OT, but AVP can stimulate uterine strips, an effect that is partially reversible by the V1 antagonist d(CH2)5TyrMeAVP. In vagina only AVP is effective in inducing contractions at nanomolar concentrations. These results suggest a role for AVP as well as OT in regulation of the motility of female genital tract.
鉴于在雄性生殖道（猪）中存在不同的催产素（OT）和加压素（VP）受体，我们重新研究了经典OT靶组织——雌性生殖道（兔）中的OT和AVP受体。使用定量配体结合、腺苷酸环化酶和收缩性研究，对阴道、子宫肌层和输卵管中的神经垂体激素受体进行了研究。我们的结果清楚地表明，子宫肌层中存在不同的OT和V1 VP受体，而在阴道和输卵管中仅检测到后者。在子宫肌层中，雌激素处理会增加OT和AVP受体的密度，而给予孕酮会抑制雌激素的作用。在自然分娩时，观察到OT位点急剧增加（17倍），而AVP位点没有变化。阴道中的AVP受体对性类固醇给药敏感，在怀孕和分娩期间会减少。等长收缩性研究表明，不仅OT，而且AVP都可以刺激子宫条带，V1拮抗剂d(CH2)5TyrMeAVP可部分逆转这种作用。在阴道中，只有AVP在纳摩尔浓度下能有效诱导收缩。这些结果表明AVP以及OT在调节雌性生殖道的运动中发挥作用。
