一种评估杀虫剂对臭虫（温带臭虫）卵及一龄若虫药效的方法。
A Method for Evaluating Insecticide Efficacy against Bed Bug, Cimex lectularius, Eggs and First Instars.
作者信息
Campbell Brittany E, Miller Dini M
机构信息
Department of Entomology and Nematology, University of Florida;
Department of Entomology, Virginia Tech.
出版信息
J Vis Exp. 2017 Mar 15(121):55092. doi: 10.3791/55092.
Standard toxicity evaluations of insecticides against insect pests are primarily conducted on adult insects. Evaluations are based on a dose-response or concentration-response curve, where mortality increases as the dose or concentration of an insecticide is increased. Standard lethal concentration (LC50) and lethal dose (LD50) tests that result in 50% mortality of a test population can be challenging for evaluating toxicity of insecticides against non-adult insect life stages, such as eggs and early instar or nymphal stages. However, this information is essential for understanding insecticide efficacy in all bed bug life stages, which affects control and treatment efforts. This protocol uses a standard dipping bioassay modified for bed bug eggs and a contact insecticidal assay for treating nymphal first instars. These assays produce a concentration-response curve to further quantify LC50 values for insecticide evaluations.
杀虫剂对害虫的标准毒性评估主要针对成虫进行。评估基于剂量反应或浓度反应曲线，即随着杀虫剂剂量或浓度的增加，死亡率也会上升。对于评估杀虫剂对非成虫生命阶段（如卵、早期幼虫或若虫阶段）的毒性而言，导致测试种群50%死亡率的标准致死浓度（LC50）和致死剂量（LD50）测试可能具有挑战性。然而，这些信息对于了解杀虫剂在臭虫所有生命阶段的功效至关重要，而这会影响控制和处理工作。本方案采用了针对臭虫卵改良的标准浸渍生物测定法以及用于处理一龄若虫的接触式杀虫测定法。这些测定法会生成浓度反应曲线，以进一步量化用于杀虫剂评估的LC50值。
Zotero 插件