Limeiras S M A, Oliveira B C, Pessatto L R, Pesarini J R, Kassuya C A L, Monreal A C D, Cantero W B, Antoniolli-Silva R, Antoniolli-Silva A C M B, Stefanello M E A, Oliveira R J
Centro de Estudos em Célula Tronco, Terapia Celular e Genética Toxicológica, Hospital Universitário Maria Aparecida Pedrossian, Empresa Brasileira de Serviços Hospitalares, Campo Grande, MS, Brasil.
Programa de Pós-Graduação em Saúde e Desenvolvimento na Região Centro-Oeste, Faculdade de Medicina "Dr. Hélio Mandetta", Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil.
Genet Mol Res. 2017 Mar 16;16(1):gmr-16-01-gmr.16019518. doi: 10.4238/gmr16019518.
The objective of this study was to evaluate the effect of Moquiniastrum polymorphum ssp floccosum ethanolic extract (MPEE) on 1,2 dimethylhydrazine (DMH)-induced colorectal carcinogenesis in mice. Forty-two male Swiss mice (Mus musculus) were subdivided into six groups (N = 7/group): negative control, DMH, MPEE, pre-treatment, simultaneous, and post-treatment. Results showed that MPEE has antigenotoxic potential on the tested protocols pre- and silmultaneous treatment, and the percent damage reductions (%DRs) were 81.88 and 93.12%, respectively. The micronucleus test demonstrated that MPEE has great antimutagenic activity, with %DRs higher than 77.09 in the associated groups. The aberrant crypt focus assay demonstrated anticarcinogenic potential of MPEE as the associated groups showed %DRs that ranged from 62.13 to 95.14%. The study shows that MPEE is nontoxic and has chemopreventive and anticarcinogenic activity, thus it may prove to be a promising medicinal plant in view of its demonstrated properties.
本研究的目的是评估多花娃儿藤亚种绵毛娃儿藤乙醇提取物（MPEE）对1,2 - 二甲基肼（DMH）诱导的小鼠结直肠癌发生的影响。42只雄性瑞士小鼠（小家鼠）被分为六组（每组N = 7）：阴性对照组、DMH组、MPEE组、预处理组、同时处理组和后处理组。结果表明，MPEE在预处理和同时处理方案中对受试方案具有抗遗传毒性潜力，损伤减少百分比（%DRs）分别为81.88%和93.12%。微核试验表明，MPEE具有很强的抗诱变活性，相关组的%DRs高于77.09%。异常隐窝灶试验表明MPEE具有抗癌潜力，因为相关组的%DRs范围为62.13%至95.14%。该研究表明，MPEE无毒，具有化学预防和抗癌活性，因此鉴于其已证明的特性，它可能被证明是一种有前途的药用植物。
