Wang Xin, Wu Chang-Xian, Song Xian-Rong, Chen Huan-Chun, Liu Zheng-Fei
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Virology. 2017 Jun;506:92-98. doi: 10.1016/j.virol.2017.03.013. Epub 2017 Mar 28.
Pseudorabies virus (PRV) China reference strain Ea is genetically closely related to newly emerged variants; however, there is limited information about PRV Ea. Here, we compared PRV Ea with new variant strains by growth kinetics, genome sequencing, and protein expression analysis. Growth analysis showed that strain Ea forms smaller plaques than strain HNX. The full-length genome sequence of Ea revealed that it is clustered in the same subgroup as HNX. Ea and HNX strains exhibited similar extracellular virion protein polymorphisms, whereas strain Bartha expressed less VP26 and more GAPDH. In infected cells, strain Ea expressed high levels of IE180 protein, and Ea and HNX produced higher levels of UL21 protein than strain Bartha. These findings provide evidence that PRV China reference strain Ea is genetically closely related to the newly emerged variant strains, indicating that strain PRV China may have evolved independently leading to the emergence of a variant strain.
伪狂犬病病毒（PRV）中国参考毒株Ea与新出现的变异株在基因上密切相关；然而，关于PRV Ea的信息有限。在此，我们通过生长动力学、基因组测序和蛋白质表达分析，将PRV Ea与新变异株进行了比较。生长分析表明，Ea毒株形成的蚀斑比HNX毒株小。Ea的全长基因组序列显示，它与HNX聚集在同一亚组中。Ea和HNX毒株表现出相似的细胞外病毒粒子蛋白多态性，而Bartha毒株表达的VP26较少，GAPDH较多。在感染细胞中，Ea毒株表达高水平的IE180蛋白，并且Ea和HNX产生的UL21蛋白水平高于Bartha毒株。这些发现提供了证据，证明PRV中国参考毒株Ea与新出现的变异株在基因上密切相关，表明中国PRV毒株可能独立进化导致了变异株的出现。
